Primary Antibodies

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Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).Adult rat hippocampus immunofluorescence under standard protocol (left) and with antigen retrieval via pepsin pretreatment (right).
Adult rat cerebellum immunohistochemistry.Adult rat brain membrane immunoblot.
Immunofluorescence staining of adult wild-type (WT) and Kv4.2 knockout (Kv4.2-/-) mouse hippocampus with K57/1 (red) and Kv2.1 rabbit (green).Adult rat brain immunohistochemistry.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot versus adult rat brain membranes (RBM), and adult mouse hippocampal membranes (MBM) from Kv3.1/Kv3.3 double knockout and WT mice (mouse samples courtesy of Dr. Rolf Joho).
Immunoblot against crude C. elegans worm extracts and crude brain membranes from adult rat (RBM) probed with K28/86 TC supe.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.Immunofluorescence of cultured hippocampal neurons with N87/25 (red) and GABA-A-R-Gamma2 rabbit polyclonal (green). Images courtesy of Angel de Blas (University of Connecticut)..
Adult rat brain hippocampus immunohistochemistry.Immunoblot against crude C. elegans worm extracts and crude brain membranes from adult rat (RBM) probed with K20/78 TC supe.
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Adult rat hippocampus CA1 immunohistochemistry.Adult rat medial vestibular nucleus immunohistochemistry.
adult rat brain membrane immunoblot immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).
Electron micrograph shows K75/41 immunolabelling in the hippocampus using a pre-embedding immunogold method. Immunogold particles (arrows) were observed along the extrasynaptic plasma membrane of dendritic shafts (Den) of interneurons establishing synaptic contacts with excitatory axon terminals (b). Scale bar = 500 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).Adult rat hippocampus immunohistochemistry.
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).Adult rat spinal cord dorsal horn immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus adult rat brain membranes (RBM), and adult mouse hippocampal membranes (MHM) from SAP102 knockout and WT mice (mouse samples courtesy of Dr. Richard Huganir).
Adult rat retina membrane immunoblot (left) and immunofluorescence (right). Green = N114/10 staining of the inner nuclear, inner plexiform, ganglion cell and optic fiber layers (INL, IPL, GCL, and OFL), where layers are viewed by differential interference contrast microscopy. Data courtesy of Gloria Partida, Tyler Stradleigh, and Andrew Ishida (UCDavis).Adult rat thalamus immunohistochemistry.
Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.

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