Coating buffers are essential solutions utilized in immunoassays, particularly in techniques like ELISA, where the effective binding of antibodies or antigens to solid surfaces is critical. These buffers are meticulously formulated to optimize the adsorption process, ensuring maximal efficiency and stability of the immobilized molecules.
Coating buffers play a crucial role in stabilizing the tertiary structures of the immobilized antibodies or antigens, thereby preserving their functionality and enhancing the specificity of the assay.
Additionally, coating buffers contribute to extending the shelf-life of coated plates by providing optimal storage conditions that prevent degradation or denaturation of the immobilized molecules over time. This ensures the reliability and reproducibility of assay results, even when plates are stored for extended periods.
ICT’s Coating Buffers enhance adsorption of antibodies or antigens onto ELISA plates, stabilize tertiary structures of adsorbed antibodies and antigens, increase shelf-life of coated plates and use less capture reagent.