Aves Labs

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Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.Representative section of formalin fixed, paraffin-embedded rat brain showing staining of GFAP. The sections were stained with Aves Labs anti-GFAP antibody (visualized in green) (Cat. No. GFAP) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.IMCD3 cells transfected with Arl13b-mCherry​ and stained  with Anti-mCherry Antibody (cat. MCHERRY, 1:300) and visualized​ with A594 anti-chicken secondary antibody. ​ As expected, cytoplasmic and ciliary signals are observed in transfected cells.​ Image kindly provided by Svetlana Makova, Yale University.
Aves Labs Anti-mCherry Antibody
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BlokHen® Blocking Reagent
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Aves Labs Anti-Iba1/AIF1 Antibody
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Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.
Aves Labs Anti-Nestin Antibody
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Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing nuclear staining of OLIG2 positive cells within the white matter of cerebellum as expected. Inset (top left) shows higher magnification. Sections were stained with AvesLabs chicken anti-OLIG2 antibody at 1:1,000 dilution and detected with anti-chicken HRP.
Aves Labs Anti-Olig2 Antibody
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Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Western blot of HIF1α immunoprecipitated, using Aves Lab's PrecipHen® (cat. P-1010), from brain lysates of fish exposed for 6 h to normoxia (>7 mg O2 l−1; lanes 2, 5, 8, 10, and 11) or hypoxia (∼1 mg O2 l−1; lanes 3-4, 6-7, and 9). A positive HIF1α control is shown in lane 1 and the mobility of HIF1α and chicken IgY are shown by arrows (at left). HIF1α protein abundance in each sample was expressed as the ratio of the HIF1α band intensity to the IgY band intensity. Image CC-BY-4.0. PMID:38116983
Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).

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