Anti-VAChT Recombinant Chicken Chimeric mAb Biotin Conjugate (N425/45)

Name: Anti-VAChT Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-429-BTN
Price: 562.0 USD
Availability: InStock

Our chicken VAChT biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone N425/45. It detects mouse and rat VAChT and is purified by affinity chromatography. Works in ELISA, IHC, ICC, WB.

Anti-VAChT Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-429-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

VAChT

Scientific Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N425/45

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc18a3 Vacht Vat

Molecular Weight

60 kDa

Immunogen

Fusion Protein Amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Biotin

Specificity

Does not cross-react with VMAT1 or VMAT2

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

VACht; Vesicular acetylcholine transporter; rVAT; Solute carrier family 18 member 3

UniProt ID

Q62666
UniProt Summary: Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis. Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern. Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation. Can transport serotonin.

NCBI Gene ID

60422

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Shipped on ice packs

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Anti-VAChT Recombinant Chicken Chimeric mAb Biotin Conjugate (N425/45)

SKU: 78-429-BTN

Product Details

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