ImmunoChemistry Technologies

303 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 1 - 24 of 303 products

Figure 1. THP-1 cells were treated with either a negative control (A), or PMA (5 ng/mL) followed by LPS (10 ng/mL). Cells were then stained with FAM-YVAD-FMK (cat. 97/98) and analyzed by phase contrast and fluorescence microscopy. In the treated sample, many cells appear bright green, indicating increased caspase-1 activity (B). In the non-induced sample, few green cells are visible, indicating a low level of caspase-1 activity (A). Data courtesy of Dr. Brian Lee, ICT (207:11).

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-1 (YVAD) Assay Kit

From $234

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin B Assay Kit

From $206

ImmunoChemistry Technologies Immulon® 2 HB 96-Well Microtiter EIA Plate

$7.30

Figure 1. Stabilization of HRP conjugates

Figure 2. Minimizes background in mono-poly sandwich ELISAs

ImmunoChemistry Technologies HRP Conjugate Stock Stabilizer, 5X

From $68.25

Figure 1. Equalize the matrix with an assay diluent

ImmunoChemistry Technologies General Assay Diluent

From $57.50

Figure 1. Signals leading to activation of the NLRP3 inflammasome.

Figure 2. C2BBe1 cells were infected with wild type Salmonella constitutively expressing mCherry (red). After 9 hours, live cells were incubated with FAM-YVAD-FMK (green cell) for 1 hour. The infected cell (red) is undergoing pyroptosis, and shows increased green fluorescence compared to uninfected cells due to caspase-1 activation. Data courtesy Knodler, Leigh A., et al. Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. PNAS. 107:41, 17733-17738 (2010).

ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Green Fluorescent

From $340.50

ImmunoChemistry Technologies Monster Block ELISA Blocking Buffer

From $52.25

ImmunoChemistry Technologies ELISA Wash Buffer, 10X

From $44.75

ImmunoChemistry Technologies TMB 1-Component HRP Microwell Substrate (SUBT)

From $44.80

ImmunoChemistry Technologies Block Buffer Optimization Pack

$217

Figure 1. Use of General Block ELISA Blocking Buffer in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies General Block ELISA Blocking Buffer

From $45.60

Figure 1. Use of ELISA Blocking Buffer - Non-Mammalian-based in Antigen-Down and Sandwich ELISA formats

ImmunoChemistry Technologies ELISA Blocking Buffer - Non-Mammalian-based

From $54.25

Figure 1. MR-(DEVD)2 (cat. 936) and Hoechst 33342 were used to quantify apoptosis in human PASMCs. Cells were treated with a negative control (A), a drug that inhibits proliferation (B), or H2O2 to induce apoptosis (C). Apoptotic cells stained red with cleaved MR-(DEVD)2 and blue with Hoechst. They have less intense blue nuclei than healthy cells, which have bright blue nuclei. Research of Dr. F. Perros, et al., Universite Paris-Sud 11; Clamart; Hopital Henri-Mondor, Creteil, France.

Figure 2. THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

ImmunoChemistry Technologies Hoechst 33342 Fluorescent Nucleic Acid Stain

$22.75

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Flow cytometry analysis of Jurkat cells treated with FLICA® 660-YVAD-FMK. After spiking FLICA® into the cell culture media, the cells were treated with either a negative control (left) or nigericin (20 μM, no.6698) to induce caspase-1 activity (right) for 24 hours. The negative control induced caspase-1 activity in 6.8% of cells (left); treatment with nigericin induced caspase-1 activity in 57.3% of cells (right). This is a ratio of 8:1. Data courtesy of Mrs. Tracy Murphy, ICT, 230-01.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit

$256.50

Figure 1. Flow cytometry analysis of active caspase-9 in MDA-MB-231 (A,B) and ZR-75-1 (C,D) cells. Cells were stained with FAM-FLICA® Caspase-9 reagent. From Figure 5 of R. Krętowski and M. Cechowska-Pasko, The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells, Int J Mol Sci (2022) 23, 9285. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 by the authors.

Flow cytometry analysis of the caspase-9 activity (Assay Kit, Cat no. 912) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

Figure 1. Using FAM-FLICA®, non-apoptotic cells (unstained, left of histogram) can easily be distinguished from apoptotic cells exhibiting green fluorescence (right of histogram). Jurkat cells were either treated with DMSO as control (A), or 1 µM staurosporine (B) to induce apoptosis. Cells were stained with FAM-FLICA® and analyzed using a flow cytometer in FL-1. Only 7% of non-induced cells (A, right) are apoptotic compared with 96.5% of the induced cells (B, right). (ICT 226:17-19.)

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were either untreated (A), or treated with 90% ethanol for 60 seconds, then stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Necrosis vs Apoptosis Assay Kit

From $340

ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test

From $173

ImmunoChemistry Technologies Foil Storage Bags for ELISA Plates

$7.80

ImmunoChemistry Technologies TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)

From $46.75

Figure 1. Dilute samples within the detection limit of the ELISA

ImmunoChemistry Technologies General Serum Diluent

From $57.25

Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 µM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-8 (LETD) Assay Kit

From $216

Figure 2. MCF-7 cells were exposed to 0.15 µM camptothecin (cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 µg/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.

ImmunoChemistry Technologies Magic Red® Fluorescent Caspase-3/7 Assay Kit

From $199.75

Figure 1. Cell death in primary rat hippocampal neurons. (A) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (D). DNA is labeled in 4 cells (D), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.

Figure 2. Normal (A) and keratoconus (B) corneal fibroblasts were treated with 200 μM H2O2 for 1 hour, washed, and allowed to recover. Cells were then treated with FAM-DEVD-FMK (cat. 93/94) for 1 hour, then washed with 1X Apoptosis Wash Buffer. Keratoconus corneal fibroblasts treated with H2O2 (B) show a significant increase in caspase-3/7 activity compared to normal cells (A). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, University of California, Irvine.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $216.25

ImmunoChemistry Technologies Streptavidin/Biotin Blocking Kit Set

From $74.50

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