Hybridoma mAb Scale-Up

Once a mouse monoclonal cell line has been sub-cloned to monoclonality, it can be used as an infinite source of monoclonal antibody (mAb) through either in vitro (bioreactor) or in vivo (ascites) production.

Method Overview: In Vitro Bioreactor Production

Cell-line Expansion (1-2 weeks)

  • Monoclonal cell line is thawed and expanded for seeding bioreactor.
  • Cell-line adaptation to serum-free medium available upon request (adaptation will take up to 3 additional weeks).

Bioreactor Production (2 weeks)

  • Cell line is seeded into bioreactor and antibody produced.

mAb Purification

 

Method Overview: In Vivo Ascites Production

Cell-line Expansion (Project-dependent timeline)

  • Monoclonal cell line is thawed and expanded for seeding mice.

Inoculation (3-4 weeks)

  • Mice are primed, cells seeded, then antibody harvested.

mAb Purification (3 weeks)

 

Ready to Get Started?

For questions or a quote from one of our scientists, submit your project details below. We will respond within two business days. If you require immediate assistance, call us at (800) 824-8540.

Thanks for your inquiry. The information you submitted has been sent to our Antibody Services Group. Typically, the team reviews submitted requests for 1-2 business days before responding so that we may offer substantial guidance from our first contact. If you need an immediate response, feel free to contact us at 1 (800) 824-8540. We look forward to working with you!
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