Anti-VGAT Recombinant Chicken Chimeric mAb Biotin Conjugate (L118/80)

Name: Anti-VGAT Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-457-BTN
Price: 562.0 USD
Availability: InStock

Our chicken VGAT biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone L118/80. It detects mouse and rat VGAT and is purified by affinity chromatography. Works in ELISA, ICC, IHC, WB.

Anti-VGAT Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-457-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

VGAT

Scientific Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

L118/80

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc32a1 Vgat Viaat

Molecular Weight

50 kDa

Immunogen

Fusion Protein Amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L118/80 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L118/80 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:100
ICC: 1:100

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

bA122O1.1 antibody; Vesicular inhibitory amino acid transporter; GABA and glycine transporter antibody; GABA and glycine transporter; hVIAAT antibody; Solute carrier family 32 member 1; SLC32A1 antibody; Vesicular GABA transporter; SLC32A 1 antibody; mVGAT; mVIAAT; solute carrier family 32 (GABA vesicular transporter member 1 antibody; Solute carrier family 32 member 1 antibody; Vesicular GABA Amino Acid Transporter antibody; Vesicular GABA transporter antibody; Vesicular inhibitory amino acid transporter antibody; VGAT antibody; VIAAT antibody; VIAAT_HUMAN antibody

UniProt ID

O35633
UniProt Summary: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.

NCBI Gene ID

22348

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Anti-VGAT Recombinant Chicken Chimeric mAb Biotin Conjugate (L118/80)

SKU: 78-457-BTN

Product Details

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