Our ELISA technical notes provide best practices for assay optimization, troubleshooting, and performance enhancement across research applications, including assay validation and data analysis. Our technical note topics also include quantification of mBDNF and proBDNF isoforms and techniques such as acid extraction of BDNF from brain tissue.
ELISA Kits Technical Notes
ELISA Assay Validation
Parallelism, linearity-of-dilution and spike-and-recovery experiments are essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.
ELISA Data Analysis
Choosing an appropriate non-linear calibration curve regression model is essential for accurate ELISA data analysis because immunoassays usually do not follow a linear dose-response relationship.
Guide to Flow Cytometry Design
Planning a flow cytometry experiment requires knowledge about the protein target of interest, selection of the correct antibody and appropriate experimental controls.
Acid Extraction of BDNF from Brain Tissue
Acid extraction can be used as an alternative tissue extraction method to release BDNF, which can be bound to its receptors and chaperons within many tissues. Samples prepared following this protocol can be assayed for BDNF content by ELISA and also used in Western Blotting to assess BDNF protein expression.
Mature BDNF Isoform Detection and Quantification
The detection and quantification of mature BDNF by ELISA poses a challenge due to potential cross-reactivity of the proBDNF full length isoform. This technical note demonstrates our efforts in validating mature BDNF quantification by ELISA, and explores the issues relating to using other immunological techniques for validation of BDNF isoform detection by ELISA assay.
proBDNF Isoform Detection
and Quantification
Less is known about full length proBDNF its role in humans. Key challenges include specific detection (independent of mature BDNF) and accurate quantification using fit-for-purpose ELISA assays. This technical note demonstrates how we have addressed both issues for proBDNF quantification in human serum, while highlighting common pitfalls with other commercially available BDNF ELISA kits.
Calculator
Quantification of Antigen Amount in Tissues and Lysates
Need a hand in calculating antigen amount in tissues homogenates or cell lysates? Make sense of your ELISA data and get your results publication ready with this simple Excel spreadsheet-based calculator.
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