Primary Antibodies

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Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
NeuroMab Anti-PSD-95 Antibody
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Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
NeuroMab Anti-Arl13b Antibody
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A microscopy image of a cell from BJ hTERT cell line treated with aphidicolin to induce genomic instability. Immunolabeling of the cell positively labelling CREST/Centromere Protein (red, 1:500, Cat No. 15-234) and labelling DNA with DAPI. The anaphase cell has a segregation error which lacks centromeric staining unlike the mass of properly segregating chromosomes. Image kindly provided by Nadeem Shaikh and Sarah McClelland from Barts Cancer Institute, Queen Mary University of London.  Confocal image of kinetochore-microtubule attachments in mouse control (CON) and IMQ treated oocytes where kinetochores are dyed purple by CREST antibody, microtubules are stained green by the anti-tubulin antibody. Chromosomes are dyed blue by Hoechst 33342.Image from publication CC-BY-4.0. PMID: 35589679
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-RGS14 Antibody
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immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
NeuroMab Anti-GFAP Antibody
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Transfected cell immunofluorescence: COS cells expressing GFP. Blue = Hoechst nuclear stain, Green = GFP, Red = N86/8.Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/8 TC supe.
NeuroMab Anti-GFP Antibody
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Immunofluorescence staining of adult rat cortex with N419/40 (red), K89/34 (green) and Hoechst nuclear stain (blue.Immunoblot against fractions of adult rat brain membranes (RBM) and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid probed with N419/40 (left) or K58/35 (right) TC supe.
Immunoblot against extracts of COS cells transfected with untagged VGlut or Kv2.1 plasmid and probed with N28/9 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
NeuroMab Anti-VGlut1 Antibody
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Adult rat hippocampus immunohistochemstry.Adult rat brain membrane immunoblot.
Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).Adult rat cerebellum immunohistochemistry.
Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.Immunofluorescence staining of cultured dorsal root ganglia neurons from Nav1.8 WT and KO mice with N134/12 TC supe. Image courtesy of Dr. Joel Black, Dr. Sulayman Dib-Hajj and Dr. Steve Waxman, Yale University.

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