PhosphoSolutions

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Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
PhosphoSolutions Anti-RBPMS Antibody
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Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Effects of striatal slice treatment with NMDA (25 μM, 5 min), dopamine (DA, 10 μM, 15 min), or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4.0. PMID: 35835216
PhosphoSolutions Anti-GluR1-Subunit (Ser845) Antibody
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Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
PhosphoSolutions Anti-NCC (Thiazide Sensitive NaCl Cotransporter) (Thr53) Antibody
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Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
PhosphoSolutions Anti-CREB (Ser133) Antibody
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Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.Immunostaining of fibrotic mouse lung tissue showing specific staining of collagen I molecules (cat. 621-COLP, 1:100, red) that are still associated with the cells in which they were synthesized. The blue is staining DNA.
PhosphoSolutions Anti-Collagen 1, alpha 1 propeptide Antibody
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Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.
PhosphoSolutions Anti-Tyrosine Hydroxylase (Ser40) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Alpha Synuclein (Ser129) Antibody
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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
PhosphoSolutions Anti-Dopamine Transporter, C-Terminus Antibody
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Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
PhosphoSolutions Anti-ERK/MAPK (Thr202/Tyr204) Antibody
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Immunohistochemical staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein (red, 1;100). Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.
PhosphoSolutions Anti-ABCA4 (Rim Protein) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-GluR1-Subunit (Ser831) Antibody
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Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
PhosphoSolutions Anti-Collagen 1, alpha 1 telopeptide Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
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Western blot of mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the ~50 kDa δ-subunit of the GABAA-R.Immunostaining of a novel ChAT-δ knock down mouse brain labeling GABAA(δ)R (Cat no 868A-GDN, 1:50, green) in WT c57Bl/6 mouse brain, and confirmation of a negative signal in the ChAT-δ knock down mouse brain. Image from publication CC-BY-4.0. PMID: 37085567
PhosphoSolutions Anti-GABAA Receptor δ, N-Terminus Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1472) Antibody
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Immunostaining of a frozen section of paraformaldehyde-fixed mouse brain showing specific immunolabeling of DDC (1:500) in red and fluorescent Nissl (blue). Photo courtesy of Tom Finger, University of Colorado School of Medicine. Western blot of rat adrenal medulla showing specific immunolabeling of the ~55 kDa DDC protein.
PhosphoSolutions Anti-DOPA Decarboxylase Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
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Western blot of HEK293 lysate showing specific immunolabeling of the ~160 kDa MerTK phosphorylated at Tyr749/753/754 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 60 minutes).
PhosphoSolutions Anti-MerTK (Tyr749/753/754) Antibody
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Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat. 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
PhosphoSolutions Anti-Olig2 Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-CaM Kinase II (Thr286) Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.
PhosphoSolutions Anti-NMDA NR2A Subunit (100 ul) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT. Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Western blot of rat kidney lysate showing specific immunolabeling of the ~24 kDa TFAM protein. Western blot of 143B cells showing specific immunolabeling human TFAM (top row). Knockout validation is demonstrated in lane 2 where cells transfected with altered TFAM via GeneSwap show no immunolabeling. Image from publication CC-BY-4.0. PMID: 35883613
PhosphoSolutions Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody
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