Anti-TMEM119 Recombinant Chicken Chimeric mAb Biotin Conjugate (L128/43)

Name: Anti-TMEM119 Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-512-BTN
Price: 562.0 USD
Availability: InStock

Our chicken TMEM119, OBIF biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone L128/43. It detects mouse TMEM119, OBIF and is purified by affinity chromatography. Works in ELISA, ICC, IHC, WB.

Anti-TMEM119 Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Mouse

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-512-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

TMEM119, OBIF

Scientific Background

TMEM119, is a plasma membrane protein first identified because of its role in the differentiation of osteoblasts (Kanamoto et al., 2009). However TMEM119 is also known to be a specific marker of microglia, the tissue-resident macrophages of brain and spinal cord (Bennett et al., 2016). Unlike other microglia markers, TMEM119 expression readily distinguishes resident microglia from blood-derived macrophages, making it a highly specific microglia marker. In healthy brain microglia are the resident immune cells where they act to neutralize pathogens, clear dead and dying cells, and prune synaptic connections. However, in neurodegenerative disease microglia can actually promote neuronal degeneration through reduced phagocytic clearance and increased pro-inflammatory effects. Our anti-TMEM119 antibody is a highly specific microglial marker for mouse TMEM119 and works by western blot and immunocytochemistry.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

L128/43

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

TMEM119

Molecular Weight

30 kDa

Immunogen

Fusion Protein Amino acids 113-280 (cytoplasmic C-terminus) of mouse Tmem119

Immunogen Species

Mouse

Species Reactivity

Mouse

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L128/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L128/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

AW208946; TMEM119; obif; transmembrane protein 119; osteoblast induction factor

UniProt ID

Q8R138
UniProt Summary: Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.

NCBI Gene ID

231633

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Anti-TMEM119 Recombinant Chicken Chimeric mAb Biotin Conjugate (L128/43)

SKU: 78-512-BTN

Product Details

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