Blocking Reagents are used to prevent non-specific binding in various biochemical assays, including immunoprecipitation, Western blotting, and immunohistochemistry. These reagents block free sites that might otherwise bind non-specifically to antibodies or other detection reagents.
Our proprietary blocking reagent, BlokHen® is a fish serum-based immunoreagent designed for use with chicken antibodies. It already contains concentrated buffer and non-ionic detergent (tween-20), allowing simple dilution with water. BlokHen® can be used in Western Blots, Immunohistochemistry or Immunocytochemistry, Immunoprecipitation, or ELISA applications.
Blocking serum serves to prevent non-specific binding of antibodies or other biomolecules to surfaces, such as cell membranes or microtiter plates. By saturating unbound sites on these surfaces, blocking serum effectively reduces background noise and enhances the specificity of assays such as immunofluorescence, enzyme-linked immunosorbent assay (ELISA), or Western blotting. These reagents help ensure accurate and reliable results by minimizing false positives and maximizing signal-to-noise ratios.