ICT’s ELISA Wash Buffer is used to wash ELISA plates between reagent addition steps. It removes unbound interfering material without compromising the positive signal. Consistently using ELISA Wash Buffer will reduce background noise, increase the specific signal, and reduce variability between assays.
ICT’s Phosphate Buffered Saline (10X PBS) is a well-tested liquid formulation of buffers and salts designed to effectively balance pH without disrupting protein binding interactions. It may be used as a base to create custom-made ELISA buffers or for other applications in the lab, such as washing cells, protein dialysis, or running Protein A or Protein G columns.