ELISA Development Kits

2 products

Showing 1 - 2 of 2 products
View
Figure 1. In Antigen-Down-ELISAs, a Target Antigen coated onto the ELISA plate surface is used to quantify specific antibody concentrations present within samples. When the sample is added, the antigen on the plate is bound by antibodies from the sample, which are then retained in the well. A species-specific antibody labeled with HRP is added, followed by substrate, a stop solution is added, and then the absorbance is read. The higher the signal, the more antibodies there are in the sample.Figure 2. Initial Assay Feasibility Plate Map. Wells are coated with 100 µL per well of either 1 µg/mL (light gray) or 4 µg/mL (dark gray) antigen coating solution, or Antigen Coating Buffer blank well controls (white). A known Positive Control is diluted out via a series of 1:2 dilutions and added to six sections of the plate. After the antibody capture step, plates are incubated with three different dilutions of the HRP-IgG conjugate, washed, and developed with TMB substrate. See protocol.
ImmunoChemistry Technologies Antigen-Down ELISA Development Kit
$572
Antibody-Sandwich ELISA Development Kit
ImmunoChemistry Technologies Antibody-Sandwich ELISA Development Kit
$649

Recently Viewed