Since the 1980s, 
has specialized in hybridoma antibody solutions tailored to meet your needs.
Monoclonal antibodies bind a single defined epitope with high specificity and can be generated against distinct epitopes in a single project. Individual clones are screened for desired characteristics. Produced by immortal hybridoma cell lines, they provide a consistent, renewable supply of antibody, while ensuring lot-to-lot consistency. Learn more about monoclonal antibodies here.
ANTIBODIES THAT WORK
- Decades of experience in hybridoma technology.
- Designed and made on-site in our Davis, CA laboratory.
- An approach that enables the discovery of antibodies that bind to a variety of epitopes.
- Optimized method for selection of immunogen and immunization schedule.
- Robust screening platform for selection of clones.
COMPREHENSIVE SERVICE
- Full-service antibodies, including: selection of immunogen, immunization schedule, and hybridoma screening approach.
- Scale it up! An optimized clone can be used as an infinite source of mAb through either in vitro (bioreactor) or in vivo (ascites) production.
- Need antibody purification or conjugation? We provide those services and more!
CUSTOMIZED TO YOUR RESEARCH
- Customer support from the scientist making your antibody.
- Our experienced team takes the time to learn your specific goals and designs an antibody solution for your research.
- Throughout your project, we work collaboratively with you to ensure the antibody meets your specifications.
Service Packages
We offer a basic monoclonal package, but additional options can be added to provide you with a fully customizable service. Contact us with your details. We look forward to providing you with a speedy project consultation and quote! See our options below:
Standard Protocol (est. 4-6 months*)
*Add 3-4 weeks if requesting peptide synthesis
*Add 8-10 weeks if requesting recombinant protein synthesis
Base Package: Custom Mouse Monoclonal Antibodies
Monoclonal production from immunization to purified antibody.
Estimated Timeframe
4-6 month production protocol
Includes:
- 5 immunized mice and serum ELISA report
- Fusion from 2 mice, >10,000 screened clones
- 20 candidates cryo-preserved, supernatent with Ab provided
- Candidates sub-cloned to monoclonality
- Bioreactor in-vitro purification
- Protein A purification (>90% pure by SDS)
- Isotyping of final purified antibody
Customized Services Available
Additional options to tailor your antibody to your unique requirements.
Immunogen Synthesis, add 1 -2 months
- Option 1: Peptide synthesis (3-4 weeks)
- Option 2: Recombinant protein synthesis (8-10 weeks)
Candidate Counterscreening (performed same day as primary screen)
- Allows for identification and removal of cross-reactive candidates.
- Required for antibodies directed against specific modifications in a protein (phosphorylation, mutations...)
Hybridoma Sequencing, add 1 week:
- Complete sequence of VH and VL domains with supporting data
Injection and Purification Timelines
VIEW Timeline Details - Mouse Monoclonals
Includes:
Immunogen Selection (Variable timeline)
- We consult with you to determine the most appropriate antigen for development of the antibodies you require.
Immunization Schedule (4 weeks)
- Immunization of 5 mice using adjuvant of choice.
- Accelerated schedule for production of high-affinity antibodies.
ELISA Titer Testing (1 week)
- ELISA testing of test bleeds to evaluate serum titer and select best-responding mouse for fusion and hybridoma creation.
Hybridoma Fusion and Screening (4-5 weeks)
- High efficiency electrofusion of spleen cells from best-responding mouse with mouse myeloma fusion partner; fusion plated across 25 x 96 well plates.
- High-throughput screening of hybridoma clones by ELISA (primary ELISA screen).
- Secondary ELISA screen on up to 94 candidate clones identified in primary screening.
- Cryo-preservation of up to 94 candidate hybridoma clones for subcloning.
- Antibody-containing supernatents made available to you for internal testing if desired.
Subcloning of Selected Clones (6 weeks)
- Selected clones sub-cloned and cell-banked.
- Monoclonality confimed by limiting dilution.
Cell-line Expansion and Bioreactor Production (3-4 weeks)
- Monoclonal cell line is thawed and expanded for seeding bioreactor.
- Cell line is seeded into bioreactor and antibody produced.
Monoclonal Antibody Purification (1 week)
- Protein A purification allows total IgG purification.
- Antibody at >90% purity in your buffer of choice.
- Isotype of purified antibody provided.
FAQs
How long will my project take?
Once we have antigen to begin immunizations it typically takes 5 months before you will have purified monoclonal antibody and clone(s) banked. If you would like us to designing and synthesizing the antigen, please add 3-4 weeks for peptide, 8-10 weeks for protein.
How much antigen should I send?
If supplying your own antigen, please provide a minimum of 1.0 mg with >90% purity preferred. If it is difficult to provide 1.0 mg or more of high purity antigen, less pure antigen can be used for immunization and higher purity antigen can be used for screening, which requires as little as 250 micrograms.
What is a hybridoma?
Hybridoma cells are formed when spleen cells isolated from the hyper-immunized mice are physically fused with cells from an immortalized mouse myeloma cell line (e.g. SP2/0 cells). This fusion process literally fuses the two different cell types to create a "hybrid" (hybridoma) cell line with nuclear DNA from both the spleen cell and the immortalized mouse myeloma "fusion partner" cell line. The antibody-secreting spleen cells isolated from the hyper-immunized mice are not immortal and will therefore senesce and die after a finite number of cell divisions. To make an immortal cell line that continues to divide and secrete the monoclonal antibody of interest, the spleen cells are 'fused' to the immortal "fusion partner" cell line, thereby creating a cell line that can grow indefinitely and continue to be a renewable source of a defined monoclonal antibody.
What is the purpose of screening?
The hybridoma fusion process creates many thousands of hybridoma cells. Screening is the process through which individual hybridoma clones secreting the most desirable antibodies are isolated from the larger pool of hybridoma candidate cells.
After the initial ELISA screen, we provide a secondary ELISA screen and cryopreservation on up to 94 candidate clones identified in primary screening
Why is subcloning necessary?
Hybridoma fusions create tens of thousands of hybridoma cells that are plated across 96-well plates and screened to identify candidates. To maximize the number of hybridoma clones screened, each individual well in a 96-well plate contains multiple individual clones. When an individual well containing a candidate is identified, cells from that well must be subjected to subcloning to ensure that only the individual clone secreting the desired antibody is isolated.
These clones are then cell banked and monoclonality is confirmed by limiting dilution.
How much antibody will I receive?
The productivity of hybridoma cell lines varies widely but generally we are able to provide 5-10 mg of monoclonal antibody and larger production amounts are available upon request.
Do you develop monoclonal antibodies against post translationally modified or mutant proteins?
Yes, we have developed many antibodies against these types of proteins. We work with you to design the right antigen for immunization, and design a screen/counterscreen strategy that will allow you to develop an antibody specific for your modification of interest.
What about intellectual property (IP) and exclusivity?
All projects are done as 'fee for service'. You retain all rights to the projects deliverables we produce for you.
Related Services
CUSTOM RECOMBINANT ANTIBODIES
- Recombinant antibodies prevent genetic drift, produce a higher yield, and can be generated in alternate species.
- Convert your favorite monoclonal antibody to a recombinant version!
CUSTOM CHICKEN POLYCLONAL ANTIBODIES
- Chicken IgY antibodies boast high yields, better specificity and easy multiplexing.
- Aves Labs has over 20 years of expertise in chicken antibody development!
CUSTOM PHOSPHOSPECIFIC ANTIBODIES
- Phosphospecific antibodies can detect the phosphorylation state of a protein.
- Our scientists are experts in phosphorylation - from peptide antigen design to purification to ELISA characterization.
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