To study the roles of the important intracellular messenger calcium in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca²⁺ detection is the use of fluorescent Ca²⁺ indicators. Fluorescent calcium and other ion indicators are powerful tools used to monitor and study the dynamics of intracellular ion concentrations. These indicators are designed to change their fluorescent properties (intensity or wavelength) upon binding to specific ions, such as calcium (Ca²⁺) or magnesium (Mg²⁺) allowing real-time monitoring of ion concentration changes in living cells.
Antibodies Inc. offers a wide variety of ion indicatiors that vary in their dissociation constants (Kd) or ion response range, excitation/emission wavelengths, spectral shift, and relative fluorescent quantum yields including Fura-2, Indo-1, Fluo-3 and Mag-Fluo-4.
