Cellular Imaging

15 products

In the pursuit of deciphering cellular phenomena, precise identification of cellular organelles such as nuclei and discerning the vitality status of cells are paramount. To facilitate this, ICT offers a diverse array of cellular imaging stains and ancillary reagents

Our catalog includes an assortment of nuclear stains, engineered to provide unparalleled clarity in delineating the boundaries of nuclei. Additionally, our range encompasses vital dyes like acridine orange, facilitating the distinction between live and dead cells, crucial for accurately interpreting experimental outcomes and ensuring data integrity.

For comprehensive cellular analysis, our selection extends to essential ancillary reagents such as gel mounting media and fixatives, meticulously formulated to preserve cellular morphology and integrity throughout the imaging process.

At ICT, we are committed to providing researchers with the tools they need for cellular exploration.

Showing 1 - 15 of 15 products

Figure 1. MR-(DEVD)2 (Cat. 936) and Hoechst 33342 were used to quantify apoptosis in human PASMCs. Cells were treated with a negative control (A), a drug that inhibits proliferation (B), or H2O2 to induce apoptosis (C). Apoptotic cells stained red with cleaved MR-(DEVD)2 and blue with Hoechst. They have less intense blue nuclei than healthy cells, which have bright blue nuclei. Research of Dr. F. Perros, et al., Universite Paris-Sud 11; Clamart; Hopital Henri-Mondor, Creteil, France.

Figure 2. THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.

ImmunoChemistry Technologies Hoechst 33342 Fluorescent Nucleic Acid Stain

$23.40

Figure 1. Propidium iodide excitation and emission spectra.

Figure 2. HL-60 cells were treated with a drug, then stained with FLICA® FAM-VAD-FMK poly-caspase inhibitor reagent (green) (Cat. 92) and propidium iodide (red) (PI). Scanning laser cytometer analysis. Four populations of cells were detected: (A) Unstained live cells, (B) red necrotic cells (PI), (C) green and red late apoptotic cells (FAM-FLICA® plus PI), and (D) green early apoptotic cells (FAM-FLICA®). Data courtesy of Dr. Z. Darzynkiewicz, Brander Cancer Center, NY.

ImmunoChemistry Technologies Propidium Iodide Stain

$23.40

ImmunoChemistry Technologies CyGEL Thermoreversible Gel

ImmunoChemistry Technologies CyGEL Sustain Thermoreversible Gel

ImmunoChemistry Technologies Col-F Collagen Binding Reagent

ImmunoChemistry Technologies DRAQ7 Far-Red Live-Cell Impermeant DNA Dye

ImmunoChemistry Technologies DRAQ5 Far-Red Fluorescent DNA Dye

Figure 2. Jurkat cells were exposed to 3% formaldehyde for 30 minutes. Following the formaldhehyde treatment, cells were stained with Green Live/ Dead stain and then imaged with a Nikon E800 microscope (DIC overlay shown). Cells with compromised membranes stained green, while cells with intact membranes excluded the Green Live/Dead stain and remained unstained. Data courtesy of Mrs. Tracy Murphy, ICT.

ImmunoChemistry Technologies Green Live/Dead Stain

$106

Immunolabeling of an organ cultured human hair follicle treated with 1.25ug/ml of ICP5249 for 5 days labeling light chain 3 I/II (green) and DAPI (Cat. 6244, blue) was used for nuclear staining. Image from publication. CC-BY-4.0 PMID: 39155393

ImmunoChemistry Technologies DAPI Nuclear Stain

$124.10

Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).

Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).

ImmunoChemistry Technologies 7-AAD Red Fluorescent Live/Dead Stain

$177.20

Figure 1. Staining of Healthy Jurkat Cells Healthy Jurkat cells were stained with CFSE or left unstained and then analyzed by flow cytometry using an Accuri C6 flow cytometer equipped with a FL1 99% attenuation filter. Unstained Jurkat cells (A), stained Jurkat cells (B), and the corresponding overlay (C) are shown.

Figure 2. Identification of CFSE-stained Cells. K562 target cells were stained green with CFSE and then subjected to effector cells. Green stained target cells are easily identifiable when analyzed by FL1 vs. SSC on a flow cytometer. Target cells stained green with CFSE move to the right along the X-axis (FL1) of the plot (right) compared with unstained effector cells. This plot becomes particularly important when gating on target cells that are the same size as effector cells.

ImmunoChemistry Technologies CFSE Green Fluorescent Stain - for cell labelling and proliferation studies

$124.40

Figure 1. Normal Jurkat cells stained with Acridine Orange (AO) show orange lysosomal staining. Cells were stained with 5 µM AO in PBS for 60 minutes at 37°C. Photomicrographs were taken using a Nikon Eclipse E800 photomicroscope using a 460-500 nm excitation filter and a 505-560 nm emission / barrier filter set at 300X. Photo B shows the corresponding DIC image of the cells in A (AO appears faintly). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Institute, New York, NY).

Figure 2. MCF-7 cells were treated with camptothecin to induce apoptosis (Cat. 6210), stained with AO in PBS, washed, and then photographed with an epifluorescence microscope at 40X using either a blue light excitation (492 nm) with a 540- 550 nm emission filter (A, lysosomes appear yellowish green), or green light excitation (540 nm) with a long pass >640 nm barrier filter (B, lysosomes appear red). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Center Institute, New York, NY).

ImmunoChemistry Technologies Acridine Orange Fluorescent Staining Solution

$23.40

ImmunoChemistry Technologies Fixative

$28.80

Figure 1. Live cells with green fluorescence (right side of histogram) can be distinguished from dead cells (unstained, left side of histogram). Forward and side scatter graphs are also shown. Jurkat cells were stained with 1 µM Calcein AM and analyzed in a flow cytometer with a FL-1 99% attenuation filter. 93.0% of the live cells stained positive with Calcein AM (A, right), while 0.0% of ethanol-killed cells stained positive (B, right). Data courtesy of Dr. Kristi Strandberg, ICT 226:87-92.

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of ethanol-treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Advanced Calcein AM Cell Viability Kit

$299

Figure 1. Live cells exhibiting green fluorescence (right of histogram) are easily distinguished from dead cells (unstained, left of histogram). Jurkat cells were either mock-treated (A) or killed by exposure to 90% ethanol (B). Cells were stained with Calcein AM, then analyzed in a flow cytometer with a FL-1 99% attenuation filter. Most (93.0%) live cells stained positive with Calcein AM (A, right), while 0.0% of the dead cells stained positive (B, right). Data courtesy of Dr. K. Strandberg.

Figure 2. Mock-treated Jurkat suspension cells were stained with Calcein AM to detect live cells. The majority of the cells imaged were considered to be live and healthy. Panel A reveals green fluorescence-stained live cells. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology. Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 226:95).

ImmunoChemistry Technologies Basic Calcein AM Cell Viability Kit

$242

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Cellular Imaging

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