Aves Labs

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Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.
Aves Labs Anti-Green Fluorescent Protein Antibody
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Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.Immunohistochemical staining of GFAP-positive radial glial cells in the periventricular zone of an e16 mouse brain. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain. 1˚ antibody (GFAP) was used at 1:500 dilution; 2˚ antibody (HRP-goat anti-chicken IgY) was used at 1:500 dilution.
Aves Labs Anti-Glial Fibrillary Acidic Protein Antibody
$285
A tissue section through an e18 mouse embryo showing β-galactosidase immunostaining (green staining) in the otic vesicle and adjacent otic ganglion. Hoechst dye (purple staining) shows the nuclei of cells.
Aves Labs Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody
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Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Aves Labs Anti-Tyrosine Hydroxylase Antibody
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Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Cat. No. F-1005, 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Aves Labs Anti-Beta-Tubulin 3 Antibody
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Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, green; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, red, 1:1000 dilution).Immunohistochemical photomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, green; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, red, 1:1000 dilution).
Aves Labs Anti-Neu-N (Fox 3) Antibody
From $135
Aves Labs Fluoresceinated Chicken Anti-Rabbit IgG Secondary Antibody
$130
Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) and Vimentin (blue) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.
Aves Labs Anti-Synaptotagmin-1 Antibody
$455
BlokHen® Blocking Reagent
Aves Labs BlokHen® Blocking Reagent
$235
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
Aves Labs Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
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Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
Aves Labs Anti-HA Epitope Tag Antibody
$295
Aves Labs Chicken Anti-Rabbit IgG Secondary Antibody
$115
Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.
Aves Labs Anti-Vimentin Protein Antibody
$455
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Aves Labs Anti-NFH (Neurofilament Heavy Chain) Antibody
$285
MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)Neonatal mouse brain cortical cultures were stained with Aves Labs MAP2 (green) and GFAP (red, rabbit antibody); Blue is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
Aves Labs Anti-Microtubule-Associated Protein Antibody
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Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.Immunoblot images of MAG and MBP (cat. MBP, 1:2000) in the somatosensory cortex of wildtype and GluD1 KO. Significant increase in MAG (***p < 0.0001, unpaired t-test) and MBP (*p < 0.0104, unpaired t-test) was found in GluD1 KO mice compared to wildtype. Image from publication CC-BY-4.0. PMID:37983226
Aves Labs Anti-Myelin Basic Protein Antibody
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A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.
Aves Labs Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody
$455
Doublecortin (1:1000 dilution, green) staining of neuroblastoma cells in culture. Red staining is a Golgi apparatus marker. Page Balisch (University of Arizona).Doublecortin (1:1000 dilution, green) staining of a tissue section (4% paraformaldehyde-fixed, paraffin- embedded) through the cochlear ganglion from a neonatal mouse. Red staining is neurofilament, NF-M, visualized with Texas Red-goat anti-rabbit IgG.
Aves Labs Anti-Doublecortin Antibody
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Amyloid Precursor Protein (APP), Peptide 3, 4, 5
Aves Labs Anti-Amyloid Precursor Protein, Peptide #3, #4, #5 Antibody
$455
Immunofluoresence of COS-7 cells expressing TPH2-flag using Aves Labs chicken anti-TPH2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-TPH2 signal and anti-flag in transfected cells.Western blotting of TPH2-flag or mock transfected COS-7 cell lysates (10 ug/ml) with Aves Labs chicken anti-TPH2 antibody (5 ug/ml) and detected with anti-mouse HRP.
Aves Labs Anti-TPH2 Antibody
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COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.Western blotting of mock or NDRG2-FLAG transfected COS-7 cell lysates and human, mouse, and rat brain homogenates (10 ug/lane) with Aves Labs chicken anti-NDRG2 antibody (0.2 ug/ml) and detected with anti-chicken HRP. Anti-NDRG2 recognizes endogenous NDRG2 in brain homogenates in addition to exogenous NDRG2 in COS-7 cells.
Aves Labs Anti-NDRG2 Antibody
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Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Aves Labs anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.
Aves Labs Anti-SOX2 Antibody
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Immunofluorescent staining of COS-7 cells expressing S100B-flag using Aves Labs anti-S100B antibody (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex). Sections were stained with Aves Labs anti-S100B antibody at 1:500 dilution and detected with anti-chicken HRP.
Aves Labs Anti-S100B Antibody
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Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing nuclear staining of OLIG2 positive cells within the white matter of cerebellum as expected. Inset (top left) shows higher magnification. Sections were stained with AvesLabs chicken anti-OLIG2 antibody at 1:1,000 dilution and detected with anti-chicken HRP.
Aves Labs Anti-Olig2 Antibody
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