Anti-Iba1/AIF1 Antibody

Name: Anti-Iba1/AIF1 Antibody
Brand: Aves Labs
SKU: IBA1-0100
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Trial Size

2 Citations

Our Anti-IBA1/AIF1 chicken primary antibody from AvesLabs is manufactured in-house. It detects IBA1/AIF1, and is purified. It is provided in liquid form.

Product Type

Primary Antibody

Applications

ICC, WB

Host Species

Chicken

Format

Affinity purified

Molecular Weight

17 kDa

Go to Full Product Details

Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.

Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.

Volume: 500 µL

Variant

Price:

$440

Quantity:

Bulk Order Anti-Iba1/AIF1 Antibody

Product Details

Target

IBA1/AIF1

Target Description

AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).

Format

Affinity purified

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Host Species

Chicken

Molecular Weight

17 kDa

Antigen

Purified recombinant human full-length IBA1/AIF1 (Uniprot P55008) expressed in E.Coli

Antibody Registry ID

AB_2910556

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

UniProt Details

UniProt (Human): P55008
UniProt (Immunogen Species): P55008

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36405424	not listed	Carpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
35633501	not listed	Galloway, D.A., et al. 2022. Investigating the NLRP3 Inflammasome and its Regulator miR-223-3p in Multiple Sclerosis and Experimental Demyelination. Journal of Neurochemistry, 94-112.

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Anti-Iba1/AIF1 Antibody

Our Anti-IBA1/AIF1 chicken primary antibody from AvesLabs is manufactured in-house. It detects IBA1/AIF1, and is purified. It is provided in liquid form.