Anti-Green Fluorescent Protein Antibody

Name: Anti-Green Fluorescent Protein Antibody
Brand: Aves Labs
SKU: GFP-1020
Price: 485.0 USD
Availability: InStock

from Aves Labs Datasheet

747 Citations

Product Type

Primary Antibody

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Molecular Weight

27 kDa

Go to Full Product Details

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.

Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.

Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.

Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.

Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.

Immunostaining of adult hippocampal neural stem cells from Nestin GFP trangenic mice labeling GFAP (orange, mouse) and GFP (orange, 1:1000, chicken, cat co GFP). The confocal image was obtained from a Leica Sp8 confocal microscope, magnification 40X. Image kindly provided by Soraya Martín Suarez, Achucarro Basque Center for Neuroscience, Spain.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Volume: 400 µL

Variant

Price:

$485

Quantity:

Bulk Order Anti-Green Fluorescent Protein Antibody

Product Details

Target

Green Fluorescent Protein (GFP)

Target Description

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format

IgY Fraction

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Molecular Weight

27 kDa

Antigen

Recombinant GFP expressed in Escherichia coli

Antibody Registry ID

AB_2307313

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product.

Shipping

Ice packs

Product Specific References for Applications and Species

FISH
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot

FISH
PMID	Dilution	Publication
37500778	1:1000	Wang, WX, et al. 2023. Universal, label-free, single-molecule visualization of DNA origami nanodevices across biological samples using origamiFISH. Nature nanotechnology, 0.

Flow Cytometry
PMID	Dilution	Publication
34732708	1:500	Jarmas, A.E., et al. 2021. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. Nature Communications, 6332.

Immunocytochemistry
PMID	Dilution	Publication
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37442236	1:2000	Dong, R, et al. 2023. The Translation Initiating Factor eIF4E and Arginine Methylation Underlie G3BP1 Function in Dendritic Spine Development of Neurons. The Journal of Biological Chemistry, 105029.
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37329884	1:1000	Livingston, NM, et al. 2023. Bursting Translation on Single mRNAs in Live Cells. Molecular Cell, 2276-2289.
37083045	1:1000	Fan, W., et al. 2023. The Transcriptional Co-Activator Yap1 Promotes Adult Hippocampal Neural Stem Cell Activation. The EMBO Journal, e110384.
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36701233	1:1000	Gallagher, E.R., et al. 2023. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Reports, 112037.
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Immunohistochemistry
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37625855	1:5000	Dos Santos, WO, et al. 2023. Growth hormone action in somatostatin neurons regulates anxiety and fear memory. The Journal of Neuroscience, .
37625412	not listed	Herriges, MJ, et al. 2023. Durable alveolar engraftment of PSC-derived lung epithelial cells into immunocompetent mice. Cell Stem Cell, .
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37620160	1:1000	Fekete, CD, et al. 2023. Cleavage of VAMP2/3 affects oligodendrocyte lineage development in the developing mouse spinal cord. The Journal of Neuroscience, .
37612280	1:500	Yao, F, et al. 2023. Deletion of ARGLU1 causes global defects in alternative splicing in vivo and mouse cortical malformations primarily via apoptosis. Cell Death & Disease, 543.
37595046	1:500	Mi, J, et al. 2023. Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish. Science Advances, eadf5142.
37590333	1:2000	Walker, LJ, et al. 2023. Target-selective vertebrate motor axon regeneration depends on interaction with glial cells at a peripheral nerve plexus. PLoS Biology, e3002223.
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37567873	1:500	Cigliola, V, et al. 2023. Spinal cord repair is modulated by the neurogenic factor Hb-egf under direction of a regeneration-associated enhancer. Nature Communications, 4857.
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37540708	1:1000	Wong, W, et al. 2023. An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold. PLoS Biology, e3002212.
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37332603	1:500	Hodge, R, et al. 2023. The Septate Junction Component Bark Beetle is Required for Drosophila Intestinal Barrier Function and Homeostasis. iScience, 106901.
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37236193	1:1000	Schneider, KM, et al. 2023. The Enteric Nervous System Relays Psychological Stress to Intestinal Inflammation. Cell, 2823-2838.
37217726	1:1000	Cury, KM, et al. 2023. Flexible Neural Control of Transition Points Within the Egg-laying Behavioral Sequence in Drosophila. Nature Neuroscience, 1054-1067.
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36964750	1:5000	de Sousa, M.E., et al. 2023. Fasting and Prolonged Food Restriction Differentially Affect GH Secretion Independently of GH Receptor Signaling in AgRP Neurons. Journal of Neuroendocrinology, e13254.
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36949723	1:500	Viana, J.F., et al. 2023. Astrocyte Structural Heterogeneity in the Mouse Hippocampus. Glia, .
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36912475	1:1000	Patel, A.R., et al. 2023. Neither GLP-1 Receptors nor GFRAL Neurons are Required for Aversive or Anorectic Response to DON (vomitoxin). American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, .
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36890237	1:1000	Bin, N.R., et al. 2023. An Airway-to-Brain Sensory Pathway Mediates Influenza-Induced Sickness. Nature, 660-667.
36889282	1:2000	Choi, M., et al. 2023. FGF21 Counteracts Alcohol Intoxication by Activating the Noradrenergic Nervous System. Cell Metabolism, 429-437.e5.
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36859533	1:400	Jiménez-Gómez, B., et al. 2023. Transgenic NADH Dehydrogenase Restores Oxygen Regulation of Breathing in Mitochondrial Complex I-Deficient Mice. Nature Communications, 1172.
36933633	1:250	Ma, R.C., et al. 2023. Origin and Diversification of Fibroblasts from the Sclerotome in Zebrafish. Developmental Biology, 35-48.
36958103	1:250	Brunswick, C.A., et al. 2023. The Clock Gene Per1 is Necessary in the Retrosplenial Cortex—but not in the Suprachiasmatic Nucleus—for Incidental Learning in Young and Aging Male Mice. Neurobiology of Aging, 77-90.
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36780219	1:1000	Hughes, B.W., et al. 2023. NPAS4 in the Medial Prefrontal Cortex Mediates Chronic Social Defeat Stress-Induced Anhedonia-Like Behavior and Reductions in Excitatory Synapses. eLife, e75631.
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Western Blot
PMID	Dilution	Publication
37596792	1:1000	Mitsou, I, et al. 2023. Two Transient Receptor Potential Channels at Focal Adhesions. The Journal of Histochemistry and Cytochemistry, 221554231194119.
37333770	1:2000	Padgett, LR, et al. 2023. Deoxyhypusine Synthase Mutations Alter the Post-translational Modification of Eukaryotic Initiation Factor 5A Resulting in Impaired Human and Mouse Neural Homeostasis. HGG Advances, 100206.
35163711	not listed	Clarke, B.E., et al. 2022. Enhanced Expression of TRAP1 Protects Mitochondrial Function in Motor Neurons under Conditions of Oxidative Stress. International Journal of Molecular Sciences, 1789.

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Anti-Green Fluorescent Protein Antibody