Anti-Glial Fibrillary Acidic Protein Antibody

Name: Anti-Glial Fibrillary Acidic Protein Antibody
Brand: Aves Labs
SKU: GFAP
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

59 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Gene Name

GFAP

Molecular Weight

50 kDa

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Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of GFAP-positive radial glial cells in the periventricular zone of an e16 mouse brain. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain. 1˚ antibody (GFAP) was used at 1:500 dilution; 2˚ antibody (HRP-goat anti-chicken IgY) was used at 1:500 dilution.

Chicken anti-Myelin Basic Protein (MBP) (green) and Mouse anti-GFAP (red) with nuclei stained with Hoechst dye (blue).

Chicken anti-NSE (green) and mouse anti-GFAP (red).

Immunostaining of rat hippocampus CA1 region identifying NeuN (red), IBA1 (green), and GFAP (Cat GFAP, 1:4000, cyan). The 30 um free-floating PFA-fixed tissue was embedded with Prolong Diamond with DAPI (grey). 10x image. Image kindly provided by Tim D. Ostrowski, A.T. Still Univ., Kirksville, MO.

Immunofluorescent staining of WT or Ncf1–/– mouse neuronal stem cells (NSCs) for Olig 4 (O4), ßIII Tubulin (Tuj1) and Gfap (cat. GFAP, 1:250). Image from publication CC-BY-4.0. PMID: 36707536

High manification view of immunofluorescent staining of WT or Ncf1–/– mouse neuronal stem cells (NSCs) for Olig 4 (O4), ßIII Tubulin (Tuj1) and Gfap (cat. GFAP, 1:250). Image from publication CC-BY-4.0. PMID: 36707536

Immunohistochemistry (Formalin-fixed paraffin-embedded) staining of adult mouse brainstem showing labeling of astrocytes with chicken anti-GFAP antibody (catalog no. GFAP) at a dilution of 1:500.

GFAP (pink, 1:500, Cat no GFAP) and DAPI (blue) staining were conducted on C57BL/6J mice at P35, two days after inducing a focal stroke in the motor cortex. The image depicts local upregulation of GFAP+ astrocytes in the motor cortex. Image kindly provided by Moawiah Naffa'A, Ph.D., Duke University, Neurobiology.

Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Volume: 200 µL

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Price:

$275

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Bulk Order Anti-Glial Fibrillary Acidic Protein Antibody

Product Details

Target

Glial Fibrillary Acidic Protein (GFAP)

Target Description

Human Glial Fibrillary Acidic Protein (GFAP) is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.

Format

IgY Fraction

Concentration

2 mg/mL (based on absorbance at 280 nm)

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

GFAP

Molecular Weight

50 kDa

Antigen

Recombinant human GFAP expressed in Escherichia coli

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2313547

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with recombinant GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Glial fibrillary acidic protein (GFAP)

Product Specific References for Applications and Species

FACS: Human
Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Human | Mouse | Non-Human Primate | Rat
Western Blot: Mouse
Additional Publications: Unspecified

FACS: Human
PMID	Dilution	Publication
28757165	1:2000	TCW, J., et al. 2017. An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Reports, 600-614.
28757163	1:1000	Ho, S.M., et al. 2017. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports, 615-628.

Immunocytochemistry: Human
PMID	Dilution	Publication
37527657	1:1000	Byun, YG, et al. 2023. Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis. Immunity, 0.
37300770	1:5000	Vulakh, G, et al. 2023. Characterizing the Neuron-Glial Interactions by the Co-cultures of Human iPSC-Derived Oligodendroglia and Neurons. Methods in Molecular Biology, 103-111.
36736291	1:1000	Linares, G.R., et al. 2023. SYF2 Suppression Mitigates Neurodegeneration in Models of Diverse Forms of ALS. Cell Stem Cell, 171-187.
30355486	1:250	Mehta, S.R., et al. 2018. Human Huntington''''s Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation. Cell Reports, 1081-1096.
29706869	not listed	Yu, Q., et al. 2018. Fibronectin Promotes the Malignancy of Glioma Stem-Like Cells Via Modulation of Cell Adhesion, Differentiation, Proliferation and Chemoresistance. Frontiers in Molecular Neuroscience, 130.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36707536	1:250	Shahin, W.S., et al. 2023. Redox-dependent Igfbp2 signaling controls Brca1 DNA damage response to govern neural stem cell fate. Nature Communications, 444.
35952494	not listed	Silva Oliveira Junior, M., et al. 2022. Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations. EBioMedicine, 104204.
31500698	not listed	Yu, H., et al. 2019. MRGPRX4 is a bile acid receptor for human cholestatic itch.. Elife, e48431.
31433979	not listed	Abdi, K., et al. 2019. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation.. Cell Reports, 2012-2022.
30314515	1:500	Putatunda, R., et al. 2018. Adult neurogenic deficits in HIV-1 Tg26 transgenic mice. Journal of Neuroinflammation, 287.
26999761	1:1000	Lassiter, C.M., et al. 2016. Embryonic stem cell-derived neural progenitors transplanted to the hippocampus migrate on host vasculature. Stem Cell Research, 579-88.

Immunocytochemistry: Rat
PMID	Dilution	Publication
29251594	1:2000	Lin, J.P., et al. 2017. LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair. eLife, e30498.

Immunohistochemistry: Human
PMID	Dilution	Publication
36303069	1:200	Leng, K., et al. 2022. CRISPRi Screens in Human iPSC-derived Astrocytes Elucidate Regulators of Distinct Inflammatory Reactive States. Nature Neuroscience, 1528-1542.
33239403	1:500	Fracassi, A., et al. 2021. Oxidative Damage and Antioxidant Response in Frontal Cortex of Demented and Nondemented Individuals with Alzheimer's Neuropathology. The Journal of Neuroscience, 538-554.
28216146	1:1000	TCW, J., et al. 2017. Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis. Stem Cell Reports, 519-528.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37558155	1:3000	Stewart, AN, et al. 2023. PTEN knockout using retrogradely transported AAVs transiently restores locomotor abilities in both acute and chronic spinal cord injury. Experimental Neurology, 114502.
37527657	1:1000	Byun, YG, et al. 2023. Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis. Immunity, 0.
37422764	1:500	Naffaa, MM, et al. 2023. Cortical regulation of neurogenesis and cell proliferation in the ventral subventricular zone. Cell reports, 112783.
37043357	1:400	Genet, N., et al. 2023. Connexin 43-Mediated Neurovascular Interactions Regulate Neurogenesis in the Adult Brain Subventricular Zone. Cell Reports, 112371.
37037362	1:1000	Abe, C., et al. 2023. Repeated Activation of C1 Neurons in Medulla Oblongata Decreases Anti-Inflammatory Effect Via the Hypofunction of the Adrenal Gland Adrenergic Response. Brain, Behavior, and Immunity, 138-150.
36941428	1:500	Chen, Z.P., et al. 2023. Lipid-Accumulated Reactive Astrocytes Promote Disease Progression in Epilepsy. Nature Neuroscience, 542-554.
36762625	1:1000	Gnanaguru, G., et al. 2023. Microglia Refine Developing Retinal Astrocytic and Vascular Networks Through the Complement C3/C3aR Axis. Development, dev201047.
36405424	1:10000	Carpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
36370710	1:500	Wang, X., et al. 2022. Driving Axon Regeneration By Orchestrating Neuronal And Non-Neuronal Innate Immune Responses Via The IFNγ-cGAS-STING Axis. Neuron, .
36303069	1:100	Leng, K., et al. 2022. CRISPRi Screens in Human iPSC-derived Astrocytes Elucidate Regulators of Distinct Inflammatory Reactive States. Nature Neuroscience, 1528-1542.
36287323	1:1000	Qu, W., et al. 2022. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice. Molecular Neurobiology, .
36258255	not listed	Ferguson, S., et al. 2022. Impact of Gulf War Toxic Exposures After Mild Traumatic Brain Injury. Acta Neuropathologica Communications, 147.
36210210	1:1000	Yamamoto-Fukuda, T., et al. 2022. Keratinocyte Growth Factor Stimulates Growth of p75+ Neural Crest Lineage Cells During Middle Ear Cholesteatoma Formation in Mice. The American Journal of Pathology, .
34471138	1:500	Ronzano, R., et al. 2021. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination. Nature Communications, 5219.
34387945	1:2000	Reitz, S.J., et al. 2021. Enhanced Multiplexing of Immunofluorescence Microscopy Using a Long-Stokes-Shift Fluorophore. Current Protocols, e214.
33728392	1:600	Ehsanipour, A., et al. 2021. Injectable, macroporous scaffolds for delivery of therapeutic genes to the injured spinal cord. APL Bioengineering, 16104.
30999016	1:500	Putatunda, R., et al. 2019. Sex-specific neurogenic deficits and neurocognitive disorders in middle-aged HIV-1 Tg26 transgenic mice.. Brain, Behavior, and Immunity, 488-499.
30472209	1:1000	Yu, F.P.S., et al. 2019. Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment. The American Journal of Pathology, 320-338.
30610918	1:1000	Dumont, C.M., et al. 2018. Aligned hydrogel tubes guide regeneration following spinal cord injury. Acta Biomaterialia, 312-322.
30465881	1:500	Hill, J.D., et al. 2018. Activation of GPR55 induces neuroprotection of hippocampal neurogenesis and immune responses of neural stem cells following chronic, systemic inflammation. Brain, Behavior, and Immunity, 165-181.
30453022	1:500	Zhang, B., et al. 2018. Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury. Brain, Behavior, and Immunity, 139-150.
30364309	1:10000	Morin, A., et al. 2018. Treatment With Nilvadipine Mitigates Inflammatory Pathology and Improves Spatial Memory in Aged hTau Mice After Repetitive Mild TBI. Frontiers in Aging Neuroscience, 292.
30314515	1:500	Putatunda, R., et al. 2018. Adult neurogenic deficits in HIV-1 Tg26 transgenic mice. Journal of Neuroinflammation, 287.
29505961	1:500	Patel, N.S., et al. 2018. Identification of new phosphodiesterase 9A (PDE9A) isoforms and how their expression and subcellular compartmentalization in brain changes across the lifespan. Neurobiology of Aging, 217-234.
29463313	1:500	Xiao, X., et al. 2018. Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells. Journal of Neuroinflammation, 49.
29379059	not listed	Yu, F.P.S., et al. 2018. Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency. Scientific Reports, 1808.
29089377	1:1000	Nakamuta, S., et al. 2017. Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain. Journal of Cell Biology, 4313-4330.
28877763	1:5000	Schweig, J.E., et al. 2017. Alzheimer's disease pathological lesions activate the spleen tyrosine kinase. Acta Neuropathologica Communications, 69.
28809400	not listed	Alachkar, A., et al. 2017. Prenatal one-carbon metabolism dysregulation programs schizophrenia-like deficits. Molecular Psychiatry, 282-294.
28456715	1:500	Szot, P., et al. 2017. Multiple lipopolysaccharide (LPS) injections alters interleukin 6 (IL-6), IL-7, IL-10 and IL-6 and IL-7 receptor mRNA in CNS and spleen. Neuroscience, 45190.
28402856	1:1000	Platt, R.J., et al. 2017. Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits. Cell Reports, 335-350.
28223982	1:2000	Ronca, S.E., et al. 2017. Mouse Model of Neurological Complications Resulting from Encephalitic Alphavirus Infection. Frontiers in Microbiology, 188.
27339393	not listed	Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice. Neuropsychopharmacology, 2920-2931.
25451251	1:1000	Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
29535375	1:500	Nagahara, A.H., et al. 2017. MR-guided delivery of AAV2-BDNF into the entorhinal cortex of non- human primates. Gene Therapy, 104-114.

Immunohistochemistry: Rat
PMID	Dilution	Publication
36521513	1:3000	Humphrey, C.M., et al. 2022. Synaptic Loss and Gliosis in the Nucleus Tractus Solitarii with Streptozotocin-induced Alzheimer's Disease. Brain Research, 148202.
34571884	not listed	Torrillas de la Cal, A., et al. 2021. The Structure of the Spinal Cord Ependymal Region in Adult Humans Is a Distinctive Trait among Mammals. Cells, 2235.
30611291	1:500	Peruzzaro, S.T., et al. 2019. Transplantation of mesenchymal stem cells genetically engineered to overexpress interleukin-10 promotes alternative inflammatory response in rat model of traumatic brain injury. Journal of Neuroinflammation, 2.
30489213	1:500	Walker, C.L., et al. 2019. Functional and Histological Gender Comparison of Age-Matched Rats after Moderate Thoracic Contusive Spinal Cord Injury. Journal of Neurotrauma, 1974-1984.
29485103	1:250	Lo, M.C., et al. 2018. Evaluating the in vivo glial response to miniaturized parylene cortical probes coated with an ultra-fast degrading polymer to aid insertion. Journal of Neural Engineering, 36002.

Western Blot: Mouse
PMID	Dilution	Publication
36287323	1:500	Qu, W., et al. 2022. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice. Molecular Neurobiology, .

Additional Publications: Unspecified
PMID	Publication
35568187	Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.
35275429	Murlanova, K., et al. 2022. Deficient mitochondrial respiration in astrocytes impairs trace fear conditioning and increases naloxone-precipitated aversion in morphine-dependent mice. Glia, 1289-1300.
30633922	Bao, Y., et al. 2019. Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways. Experimental Eye Research, 15-24.
29913164	Zhang, Y., et al. 2018. Structural and visual functional deficits in a rat model of neuromyelitis optica spectrum disorders related optic neuritis. Experimental Eye Research, 124-132.
24639124	Hasegawa, Y., et al. 2014. Spatiotemporal Distribution of SUMOylation Components during Mouse Brain Development. The Journal of Comparative Neurology Research in Systems Neuroscience, 3020-3036.

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Anti-Glial Fibrillary Acidic Protein Antibody