Aves Labs

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Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.
Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).
Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Aves Labs Anti-V5 Antibody
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Immunostaining of HeLa cells showing specific labeling of Ki-67 (Cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.
Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) and Vimentin (blue) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.
Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-TREM2 showing specific immunolabeling of the endogenous TREM2 at ~35kDa.
Aves Labs Anti-TREM2 Antibody
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Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.
Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.
Immunofluoresence of COS-7 cells expressing TPH2-flag using Aves Labs chicken anti-TPH2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-TPH2 signal and anti-flag in transfected cells.Western blotting of TPH2-flag or mock transfected COS-7 cell lysates (10 ug/ml) with Aves Labs chicken anti-TPH2 antibody (5 ug/ml) and detected with anti-mouse HRP.
Aves Labs Anti-TPH2 Antibody
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Goat Anti-Chicken IgY Secondary Antibody
Immunohistochemical presence of tau in neurofibrillary tangles in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemical presence of tau in a neurofibrillary tangle in the remnant of a cortical neuron of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.
Aves Labs Anti-Tau Antibody
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Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (Aves Cat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.
Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Calnexin (CANX) (Cat. CANX-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-Calnexin specifically stains the Endoplasmic Reticulum in HeLa cells. ​Western blotting of various cell lysates with Aves Labs chicken anti-Calnexin (CANX) antibody (0.05 µg/ml) and detected with anti-chicken HRP. Aves Labs Chicken anti-Calnexin recognizes endogenous Calnexin in all the cell lysates at ~90 kDa.
Aves Labs Anti-Calnexin Antibody
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HeLa cells were subjected to immunofluorescent staining using chicken anti-TOMM20 antibody (visualized in green) and Antibodies Inc mouse anti-Mortalin antibody (75-127) (visualized in red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (AR-6501). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mortalin antibody specifically in the cell mitochondria.Western blotting of various cell lysates with chicken anti-TOMM20 antibody (0.2 µg/ml)(1:1000) and detected with anti-chicken HRP. Chicken anti-TOMM20 recognizes endogenous TOMM20 in all the cell lysates at ~16 kDa.
Aves Labs Anti-TOMM20 Antibody
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Chicken Anti-Rabbit IgG Secondary Antibody

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