Aves Labs

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Immunofluoresence of COS-7 cells expressing TPH2-flag using Aves Labs chicken anti-TPH2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-TPH2 signal and anti-flag in transfected cells.Western blotting of TPH2-flag or mock transfected COS-7 cell lysates (10 ug/ml) with Aves Labs chicken anti-TPH2 antibody (5 ug/ml) and detected with anti-mouse HRP.
Aves Labs Anti-TPH2 Antibody
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COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.Western blotting of mock or NDRG2-FLAG transfected COS-7 cell lysates and human, mouse, and rat brain homogenates (10 ug/lane) with Aves Labs chicken anti-NDRG2 antibody (0.2 ug/ml) and detected with anti-chicken HRP. Anti-NDRG2 recognizes endogenous NDRG2 in brain homogenates in addition to exogenous NDRG2 in COS-7 cells.
Aves Labs Anti-NDRG2 Antibody
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Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Aves Labs anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.
Aves Labs Anti-SOX2 Antibody
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Immunofluorescent staining of COS-7 cells expressing S100B-flag using Aves Labs anti-S100B antibody (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex). Sections were stained with Aves Labs anti-S100B antibody at 1:500 dilution and detected with anti-chicken HRP.
Aves Labs Anti-S100B Antibody
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Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing nuclear staining of OLIG2 positive cells within the white matter of cerebellum as expected. Inset (top left) shows higher magnification. Sections were stained with AvesLabs chicken anti-OLIG2 antibody at 1:1,000 dilution and detected with anti-chicken HRP.
Aves Labs Anti-Olig2 Antibody
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Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-TREM2 showing specific immunolabeling of the endogenous TREM2 at ~35kDa.
Aves Labs Anti-TREM2 Antibody
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Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Aves Labs Anti-Iba1/AIF1 Antibody
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Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Aves Labs Anti-V5 Antibody
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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.Western blotting of recombinant fluorescent proteins with AvesLabs Chicken anti- mCherry antibody. The indicated (nanogram) amounts of each purified recombinant fluorescent protein was loaded on a gel and analyzed by Western blotting. AvesLabs Chicken anti-mCherry antibody recognizes ng amounts of mCherry protein and the related dsRED protein but does not show any reactivity with GFP.
Aves Labs Anti-mCherry Antibody
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Chicken anti-SNCA (green) and TH (red) double immunostaining in Parkinson's Disease patient’s substantia nigra section. Arrows indicate SNCA positive, degenerating dopamine neurons. Arrowheads indicate SNCA negative, healthy dopamine neurons. Bar = 50 um for A-D. (Image courtesy of Dr. Curt Freed’s lab, University of Colorado Anschutz Medical Campus.)Rat brain lysate was probed with no primary antibody as a control (Lane 1) or with a 1:2,500 dilution of Chicken anti-SNCA IgY (Lane 2).
Western blot of HIF1α immunoprecipitated, using Aves Lab's PrecipHen® (cat. P-1010), from brain lysates of fish exposed for 6 h to normoxia (>7 mg O2 l−1; lanes 2, 5, 8, 10, and 11) or hypoxia (∼1 mg O2 l−1; lanes 3-4, 6-7, and 9). A positive HIF1α control is shown in lane 1 and the mobility of HIF1α and chicken IgY are shown by arrows (at left). HIF1α protein abundance in each sample was expressed as the ratio of the HIF1α band intensity to the IgY band intensity. Image CC-BY-4.0. PMID:38116983

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