Anti-HA Epitope Tag Antibody

Name: Anti-HA Epitope Tag Antibody
Brand: Aves Labs
SKU: ET-HA100
Price: 285.0 USD
Availability: InStock

from Aves Labs Datasheet

10 Citations

Product Type

Primary Antibody

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Format

Affinity Purified IgY

Molecular Weight

N/A

Go to Full Product Details

Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.

HeLa cells transiently transfected with TFPI, TFPI2, mTFPI, SEMA6A, or FZD2 were exposed to FLAG-tagged TcdB4.21286–1805 (5 µg/mL) on ice for 60 min, washed, fixed, permeabilized, and subjected to immunostaining analysis. Expression of exogenous proteins was confirmed by detecting fused HA (cat. ET-HA) or 1D4 tag. Nuclei were labeled with DAPI (blue)

TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897

Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.

Volume: 1 mL

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Product Details

Target

HA Epitope Tag Antibody

Target Description

The synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA).

Format

Affinity Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Molecular Weight

N/A

Antigen

Synthetic peptide

Antibody Registry ID

AB_2313511

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:5000
IHC: 1:500
ICC: 1:500

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Drosophila | Mouse
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
36351897	1:1000	Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.

Immunocytochemistry: Rat
PMID	Dilution	Publication
35471151	1:1000	Wu, C.H., et al. 2022. A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling. Elife, e74277.

Immunohistochemistry: Drosophila
PMID	Dilution	Publication
26400093	1:400	Ordan, E., et al. 2015. A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning. Development , 3512-3518.
20110313	not listed	Gilsohn, E., et al. 2010. Slowdown promotes muscle integrity by modulating integrin-mediated adhesion at the myotendinous junction. Development, 785-794.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
34932948	1:100	Kato, T., et al. 2021. Dynamic stem cell selection safeguards the genomic integrity of the epidermis. Developmental cell, 3309-3320.
34582787	1:300	Wang, L.L., et al. 2021. Revisiting astrocyte to neuron conversion with lineage tracing in vivo. Cell, 5465-5481.
32641826	1:1000	Muller, P.A., et al. 2020. Microbiota modulate sympathetic neurons via a gut-brain circuit. Nature , 441-446.
32302524	not listed	Tian, S., et al. 2020. Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Cell Host and Microbe, 782-792.
26758850	not listed	Yuan, P., et al. 2016. Attenuation of β-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity. Journal of Neuroscience, 632-641.

Western Blot: Human
PMID	Dilution	Publication
36351897	1:1000	Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.

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Anti-HA Epitope Tag Antibody