Anti-HA Epitope Tag Antibody

20 Citations

Our Anti-HA Epitope Tag Antibody chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects HA Epitope Tag Antibody, and is affinity-purified IgY. It is great for use in ELISA, IHC, ICC, IP, WB.

Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.
Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
HeLa cells transiently transfected with TFPI, TFPI2, mTFPI, SEMA6A, or FZD2 were exposed to FLAG-tagged TcdB4.21286–1805 (5 µg/mL) on ice for 60 min, washed, fixed, permeabilized, and subjected to immunostaining analysis. Expression of exogenous proteins was confirmed by detecting fused HA (cat. ET-HA) or 1D4 tag. Nuclei were labeled with DAPI (blue)
TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
Representative image of astrocyte transduced with AAV, labeled with HA (cat. ET-HA100; blue) and astrocytemarker (Sox9). Neurons are identified by NeuN staining. Image from publication CC-BY-4.0. PMID:38574730
Representative images of astrocytes transduced with AAV Control-HA or AAV NCAN ELS-HA (marked with HA (cat. ET-HA100) in red) and NCAN C-terminal in cyan. Image from publication CC-BY-4.0. PMID:38574730
Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.
Roll over image to zoom in Click on image to zoom
Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 µg of protein loaded in each lane.
Immunolabeling of mouse hindbrain dorsal motor nucleus of the vagus (DMV) neurons expressing an hM3Dq-HA tag (Anti-HA Epitope Tag, Cat ET-HA100, 1:200). The image is kindly provided by Nicholas Conley, Neuroscience Graduate Program, University of Virginia.
HeLa cells transiently transfected with TFPI, TFPI2, mTFPI, SEMA6A, or FZD2 were exposed to FLAG-tagged TcdB4.21286–1805 (5 µg/mL) on ice for 60 min, washed, fixed, permeabilized, and subjected to immunostaining analysis. Expression of exogenous proteins was confirmed by detecting fused HA (cat. ET-HA) or 1D4 tag. Nuclei were labeled with DAPI (blue)
TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
Representative image of astrocyte transduced with AAV, labeled with HA (cat. ET-HA100; blue) and astrocytemarker (Sox9). Neurons are identified by NeuN staining. Image from publication CC-BY-4.0. PMID:38574730
Representative images of astrocytes transduced with AAV Control-HA or AAV NCAN ELS-HA (marked with HA (cat. ET-HA100) in red) and NCAN C-terminal in cyan. Image from publication CC-BY-4.0. PMID:38574730

ELISA, ICC, IHC, IP, WB
Chicken

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Data Sheet

SKU: ET-HA100

Volume: 1 mL
Price:
$304.00
Ships: 1-2 business days

Product Specific References for Applications and Species

ELISA: Human
PMID Dilution Publication
221229111:5000Bolton, M.J., et al. 2011. Sequence similarity between the erythrocyte binding domain of the Plasmodium vivax Duffy binding protein and the V3 loop of HIV-1 strain MN reveals a …. Virology Journal, 523.
Immunocytochemistry: Human
PMID Dilution Publication
366382141:1000Hale, W.D., et al. 2023. Engineered adhesion molecules drive synapse organization. PNAS USA, .
363518971:1000Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.
109061371:100Fan, X., et al. 2000. The α subunits of Gz and Gi interact with the eyes absent transcription cofactor Eya2, preventing its interaction with the six class of homeodomain-containing …. Journal of Biological Chemistry, 32129-32134.
Immunocytochemistry: Rat
PMID Dilution Publication
354711511:1000Wu, C.H., et al. 2022. A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling. Elife, e74277.
Immunocytochemistry: Yeast
PMID Dilution Publication
23336935not listedKaur, N., et al. 2013. Arabidopsis RING Peroxins are E3 Ubiquitin Ligases that Interact with Two Homologous Ubiquitin Receptor ProteinsF. Journal of Integrative Plant Biology, 108-120.
Immunohistochemistry: Drosophila
PMID Dilution Publication
264000931:400Ordan, E., et al. 2015. A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning. Development , 3512-3518.
20110313not listedGilsohn, E., et al. 2010. Slowdown promotes muscle integrity by modulating integrin-mediated adhesion at the myotendinous junction. Development, 785-794.
Immunohistochemistry: Mouse
PMID Dilution Publication
38574730not listedIrala, D, et al. 2024. Astrocyte-secreted neurocan controls inhibitory synapse formation and function. Neuron, 1657-1675.
384205851:200Strain, MM, et al. 2024. Dorsal motor vagal neurons can elicit bradycardia and reduce anxiety-like behavior. iScience, 109137.
349329481:100Kato, T., et al. 2021. Dynamic stem cell selection safeguards the genomic integrity of the epidermis. Developmental cell, 3309-3320.
345827871:300Wang, L.L., et al. 2021. Revisiting astrocyte to neuron conversion with lineage tracing in vivo. Cell, 5465-5481.
326418261:1000Muller, P.A., et al. 2020. Microbiota modulate sympathetic neurons via a gut-brain circuit. Nature , 441-446.
32302524not listedTian, S., et al. 2020. Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Cell Host and Microbe, 782-792.
26758850not listedYuan, P., et al. 2016. Attenuation of β-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity. Journal of Neuroscience, 632-641.
Immunoprecipitation: Mouse
PMID Dilution Publication
173810775ulHrabchak, C., et al. 2007. A testis specific isoform of endophilin B1, endophilin B1t, interacts specifically with protein phosphatase-1cγ2 in mouse testis and is abnormally expressed in PP1cγ …. Biochemistry, 4635-4644.
Western Blot: Human
PMID Dilution Publication
363518971:1000Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.
22700985not listedZhang, L.J., et al. 2012. Coordinated regulation of transcription factor Bcl11b activity in thymocytes by the mitogen-activated protein kinase (MAPK) pathways and protein sumoylation. Journal of Biological Chemistry, 26971-26988.
167697271:1000Stefansson, B., et al. 2006. Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IκBϵ. Journal of Biological Chemistry, 22624-22634.
Western Blot: Non-Human Primate
PMID Dilution Publication
181866511:1000Stefansson, B., et al. 2008. Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains. Biochemistry, 1442-1451.

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