Anti-Beta-Tubulin 3 Antibody

Name: Anti-Beta-Tubulin 3 Antibody
Brand: Aves Labs
SKU: TUJ
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Trial Size

38 Citations

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

TUBB3 TUBB4

Molecular Weight

50 kDa

Go to Full Product Details

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Cat. No. F-1005, 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000 dilution). Red staining is rabbit-anti-GFAP (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG Dr. Gerry Shaw (EnCor Biotechnology).

β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.

β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000). Hoda Ilias, Aves Labs.

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr. Gerry Shaw (EnCor Biotechnology).

Volume: 1000 µL

Variant

Price:

$440

Quantity:

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Product Details

Target

Beta-Tubulin 3

Target Description

Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

Format

Affinity-Purified IgY

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

TUBB3 TUBB4

Molecular Weight

50 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313564

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III)

UniProt Details

UniProt (Immunogen Species): Q13509

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
31743443	1:500	Candelario, K.M., et al. 2020. Exosome/microvesicle content is altered in leucine-rich repeat kinase 2 mutant induced pluripotent stem cell-derived neural cells. Journal of Comparative Neurology, 1203-1215.
31134192	1:2000	Kamande, J.W., et al. 2019. Multi-compartment Microfluidic Device Geometry and Covalently Bound Poly-D-Lysine Influence Neuronal Maturation. Frontiers in Bioengineering and Biotechnology, 84.
30430656	1:1000	Göttle, P., et al. 2018. Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination. Glia, 160-170.
28966121	not listed	Wang, C., et al. 2017. Scalable Production of iPSC-Derived Human Neurons to Identify Tau-Lowering Compounds by High-Content Screening. Stem Cell Reports, 1221-1233.
28377585	1:2000	Bigler, R.L., et al. 2017. Messenger RNAs localized to distal projections of human stem cell derived neurons. Nature Scientific Reports, 611.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36150462	1:250	Casagrande, L., et al. 2022. Moderate intestinal immunopathology after acute oral infection with Toxoplasma gondii oocysts is associated with expressive levels of serotonin. Life sciences, 120985.
35618205	1:250	Libner, C.D., et al. 2022. Autoimmunity to a ribonucleoprotein drives neuron loss in multiple sclerosis models. Neurobiology of disease, 105775.
34697074	1:500	Anees, A., et al. 2021. Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells. eNeuro, .
34679349	not listed	Li, M., et al. 2021. Pro-Inflammatory Cytokines and Antibodies Induce hnRNP A1 Dysfunction in Mouse Primary Cortical Neurons. Brain Sciences, 1282.
32273484	1:200	Bott, C.J., et al. 2020. Nestin Selectively Facilitates the Phosphorylation of the Lissencephaly-Linked Protein Doublecortin (DCX) by cdk5/p35 to Regulate Growth Cone Morphology and Sema3a Sensitivity in Developing Neurons. Journal of Neuroscience, 3720-3740.
30840538	1:200	Bott, C.J., et al. 2019. Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity.. Molecular Biology of the Cell, 1214-1229.
30185885	1:1000	Brudvig, J.J., et al. 2018. MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network. Scientific Reports, 13278.
29666444	1:500	Liu, Z., et al. 2018. Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells. Scientific Reports, 6087.
29209158	1:300	Al-Gharaibeh, A., et al. 2017. Induced Pluripotent Stem Cell-Derived Neural Stem Cell Transplantations Reduced Behavioral Deficits and Ameliorated Neuropathological Changes in YAC128 Mouse Model of Huntington''s Disease. Frontiers in Neuroscience, 628.

Immunocytochemistry: Rat
PMID	Dilution	Publication
29534752	1:1000	Göttle, P., et al. 2018. Teriflunomide promotes oligodendroglial differentiation and myelination. Journal of Neuroinflammation, 76.
29309895	not listed	Muñoz, Y., et al. 2018. Reactive oxygen species released from astrocytes treated with amyloid beta oligomers elicit neuronal calcium signals that decrease phospho-Ser727-. Free Radical Biology and Medicine, 132-144.
28931811	1:2000	Nagendran, T., et al. 2017. Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling. Natrue Communications, 625.
28669529	not listed	Nagai, T., et al. 2017. Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture. Journal of Bioscience and Bioengineering, 430-438.
26132738	1:200	Daadi, M.M., et al. 2016. Optogenetic Stimulation of Neural Grafts Enhances Neurotransmission and Downregulates the Inflammatory Response in Experimental Stroke Model. Cell Transplantation, 1371-1380.
25609610	1:1000	Göttle, P., et al. 2015. Oligodendroglial Maturation Is Dependent on Intracellular Protein Shuttling. The Journal of Neuroscince, 906-919.

Immunohistochemistry: Human
PMID	Dilution	Publication
36303071	1:1000	Oh, Y.M., et al. 2022. Age-related Huntington's Disease Progression Modeled in Directly Reprogrammed Patient-derived Striatal Neurons Highlights Impaired Autophagy. Nature Neuroscience, 1420-1433.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36370710	not listed	Wang, X., et al. 2022. Driving Axon Regeneration By Orchestrating Neuronal And Non-Neuronal Innate Immune Responses Via The IFNγ-cGAS-STING Axis. Neuron, .
34778272	1:1000	Hu, Z., et al. 2021. Generation of a Spiral Ganglion Neuron Degeneration Mouse Model. Frontiers in Cell and Developmental Biology, 761847.
32641826	1:200	Muller, P.A., et al. 2020. Microbiota modulate sympathetic neurons via a gut-brain circuit. Nature, 441-446.
30820459	1:1000	Dong, J., et al. 2019. A neuronal molecular switch through cell-cell contact that regulates quiescent neural stem cells. Science Advances, eaav4416.
30004576	1:1000	Yamada, S., et al. 2018. Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system. Journal of Comparative Neurology, 2099-2114.
29463313	not listed	Xiao, X., et al. 2018. Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells. Journal of Neuroinflammation, 49.

Western Blot: Human
PMID	Dilution	Publication
29740275	1:1000	DeVos, S.L., et al. 2018. Synaptic Tau Seeding Precedes Tau Pathology in Human Alzheimer''s Disease Brain. Frontiers in Neuroscience, 267.

Western Blot: Mouse
PMID	Dilution	Publication
32811565	Not listed	Bennett, R.E., et al. 2020. Tau reduction in aged mice does not impact Microangiopathy. Acta Neuropathologica Communications, 137.
29617675	not listed	Morton, M.C., et al. 2018. Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen. Cell Reports, 78-89.
27871935	not listed	Vuong, T.A., et al. 2016. A Sonic hedgehog coreceptor, BOC regulates neuronal differentiation and neurite outgrowth via interaction with ABL and JNK activation. Cellular Signalling, 30-40.

Western Blot: Rat
PMID	Dilution	Publication
35474277	1:250	Yap, C.C., et al. 2022. Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation. The Journal of neuroscience : the official journal of the Society for Neuroscience, 4415-4434.
30759353	1:5000	Cury, D.P., et al. 2019. Application of a Purified Protein From Natural Latex and the Influence of Suture Type on Achilles Tendon Repair in Rats. The American Journal of Sports Medicine, 901-914.
30587852	1:50000	Anderson, E.M., et al. 2019. Knockdown of the histone di-methyltransferase G9a in nucleus accumbens shell decreases cocaine self-administration, stress-induced reinstatement, and anxiety.. Neuropsychopharmacology, 1370-1376.
29907658	1:7500	Yap, C.C., et al. 2018. Degradation of dendritic cargos requires Rab7- dependent transport to somatic lysosomes. The Journal of Cell Biology, 3141-3159.
29217682	not listed	Anderson, E.M., et al. 2018. Overexpression of the Histone Dimethyltransferase G9a in Nucleus Accumbens Shell Increases Cocaine Self-Administration, Stress-Induced Reinstatement, and Anxiety. The Journal of Neuroscince, 803-813.
28874679	1:7500	Yap, C.C., et al. 2017. The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes. Scientific Reports, 10481.

Additional Publications: Unspecified
PMID	Publication
36310215	Gribaudo, S., et al. 2009. Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions. Methods in Molecular Biology, 357-378.

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Anti-Beta-Tubulin 3 Antibody

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.