Anti-Choline Acetyltransferase Antibody

Name: Anti-Choline Acetyltransferase Antibody
Brand: Aves Labs
SKU: CAT
Price: 470.0 USD
Availability: InStock

3 Citations

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (Q03059) and human (P28329) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

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Data Sheet

Trial Size

SKU: CAT

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Murine choline-O-acetyltransferase (EC 2.3.1.6)

Scientific Background

Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein (640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

CHAT

Molecular Weight

72 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313537

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: Not Recommended
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Choline O-acetyltransferase (CHOACTase) (ChAT) (Choline acetylase) (EC 2.3.1.6)

UniProt ID

P28329

Shipping

Ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Marmoset | Mouse | Rat

Immunohistochemistry: Marmoset
PMID	Dilution	Publication
33798453	1:300	Yoshimatsu, S., et al. 2021. Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species. Stem Cell Reports, 754-770.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
25921813	1:1000	Niu, W., et al. 2015. SOX2 Reprograms Resident Astrocytes into Neural Progenitors in the Adult Brain. Stem Cell Reports, 780-794.

Immunohistochemistry: Rat
PMID	Dilution	Publication
22624977	1:500	Focant, M.C., et al. 2013. PICK1 expression in reactive astrocytes within the spinal cord of amyotrophic lateral sclerosis (ALS) rats. Neuropathology and Applied Neurobiology, 231-242.

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Anti-Choline Acetyltransferase Antibody

SKU: CAT

Product Details

Product Specific References for Applications and Species

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