Anti-Cortactin Recombinant Chicken Chimeric mAb Biotin Conjugate (4F11-G5-F10)

Name: Anti-Cortactin Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-601-BTN
Price: 562.0 USD
Availability: InStock

Our chicken Cortactin biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone 4F11-G5-F10. It detects bovine, chicken, hamster, human, mouse, and rat Cortactin and is purified by affinity chromatography. Works in ELISA, IHC, ICC, WB, IP.

Anti-Cortactin Recombinant Chicken Chimeric mAb Biotin Conjugate

Click on image to zoom

Species Reactivity

Bovine, Chicken, Hamster, Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 78-601-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Cortactin

Scientific Background

Cortactin is an ~80–85 kDa actin-binding protein that orchestrates cytoskeletal remodeling and signal integration. It contains an N-terminal repeat domain that associates with filamentous actin, followed by a helical segment, a proline-rich region with multiple signaling motifs, and a C-terminal SH3 domain. Through interactions with the Arp2/3 complex and other regulators, cortactin promotes actin branching, lamellipodia formation, and endocytic trafficking. Tyrosine phosphorylation by Src family kinases inhibits its actin crosslinking activity, linking receptor-mediated signals to cytoskeletal rearrangements. Aberrant cortactin activity has been implicated in tumor cell invasion, metastasis, and vascular permeability disorders, highlighting its importance in both normal cellular dynamics and disease progression.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

4F11-G5-F10

Isotype

IgY

Applications

ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

CTTN

Molecular Weight

65 kDa

Immunogen

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Immunogen Species

Chicken

Species Reactivity

Bovine, Chicken, Hamster, Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 4F11-G5-F10 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 4F11-G5-F10 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

ICC: 1:100-1:500

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

EMS1; Amplaxin; 1110020L01Rik; oncogene EMS1; Src substrate cortactin

UniProt ID

Q01406
UniProt Summary: Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. Plays a role in the invasiveness of cancer cells, and the formation of metastases.

NCBI Gene ID

2017

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Cortactin Recombinant Chicken Chimeric mAb Biotin Conjugate (4F11-G5-F10)

SKU: 78-601-BTN

Product Details

Bulk Order Anti-Cortactin Recombinant Chicken Chimeric mAb Biotin Conjugate

Related Products

Recently Viewed