Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb Biotin Conjugate (30H3K1)

Name: Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-605-BTN
Price: 562.0 USD
Availability: InStock

Our chicken Ubiquitin (Ser65) phosphospecific biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone 30H3K1. It detects human, mouse, and rat Ubiquitin (Ser65) and is purified by affinity chromatography. KO validated. Works in ELISA, IHC, ICC, WB.

Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 78-605-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Ubiquitin (Ser65)

Scientific Background

Ubiquitin phosphorylation at ser65 (pS65-Ub) is implicated in mitochondrial quality control and the molecular origins of Parkinson’s Disease. The protein kinase PINK1 phosphorylates ubiquitin at the ser65 residue in response to mitochondrial stress. This acts as a powerful "on-switch" for the E3 ubiquitin ligase Parkin increasing its enzymatic activity and recruiting it to damaged mitochondria, initiating a specialized degradation process known as mitophagy. When mitochondria are depolarized or damaged, the accumulation of pS65-Ub triggers the clearance of these damaged organelles before they can release toxic reactive oxygen species (ROS) or pro-apoptotic factors. Genetic mutations in either PINK1 or Parkin that disrupt this phosphorylation event are a primary cause of autosomal recessive juvenile Parkinsonism.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

30H3K1

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

UBC, UBB

Molecular Weight

Multiple bands

Immunogen

The exact immunogen used to generate this antibody is proprietary information

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 30H3K1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 30H3K1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

HMG20; Polyubiquitin-C; phospho Ser65,Ser65-phosphorylated ubiquitin; Polyubiquitin-B; pSer65-Ub; Ubiquitin

UniProt ID

P0CG48
UniProt Summary: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2.
As a monoubiquitin, its C-terminal glycine is recognized as a C-degron by Cul2-RING (CRL2) E3 ubiquitin-protein ligase complexes (PubMed:39548056)

NCBI Gene ID

7316

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Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb Biotin Conjugate (30H3K1)

SKU: 78-605-BTN

Product Details

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