Antibodies for Neuroscience Research

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Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.Immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.
Adult rat brain membrane immunoblot.Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (Cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554
Adult rat cerebellar immunohistochemistry.Representative confocal images of control and α-Syn fibril-treated mouse neurons stained with IP3R1 (Cat. 75-035; magenta) and MAP2 (cyan) with or without treatment of 100 nM UNC-3230. Zoomed-in images show only IP3R1. Image from publication CC-BY-4.0. PMID:37838947
Western blot of rat cortical lysate showing specific immunolabeling of the ~48 kDa, ~65 kDa & ~75 kDa tau isoforms.Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-TAU antibody (Cat. 1998-TAU, green, 1:2000) and anti-MAP2 (red). The blue is DAPI staining nuclear DNA. Anti-TAU labels perikarya, dendrites, axons of neurons while anti-MAP2 only labels dendrites and perikarya of neurons. Where they overlap they appear orange-yellow.
PhosphoSolutions Anti-Tau Antibody
Sale priceFrom $164
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Immunoblot against crude membranes from adult rat brain (RBM), mouse brain (MBM) and wild-type (WT) and ATAT1 knockout (KO) mouse brains probed with N347/42 (left) or N52A/42 (right) TC supe. WT and KO mouse brains courtesy of Andrea Aguilar and Max Nachury (Stanford
NeuroMab Anti-ATAT1 Antibody
Sale priceFrom $142
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Beta-Amyloid-positive “neuritic plaque” (shown in higher magnification) in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer’s disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemistry of a capillary showing beta-amyloid peptide in the wall of the vessel. Paraffin-embedded section of a human brain from a patient with diagnosed Alzheimer's Disease. Primary antibody -- 1:500; secondary antibody -- 1:1000 HRP-Goat anti-chicken IgY (Aves Labs). Dr. Randy Woltjer, Dept Pathology, OHSU
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.
Anti-Navbeta1 Na+ Channel Antibody
Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.Adult rat hippocampus immunohistochemistry.
Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).Adult rat hippocampus immunohistochemistry.

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