Antibodies for Neuroscience Research

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Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (Cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218
Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.Adult rat brain membrane immunoblot.
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).Adult rat whole brain immunohistochemistry.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.
NeuroMab Anti-Olig1 Antibody
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Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids and probed with N112B/14 (left) and K89/41 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude brain membranes from adult rat (RBM) and wild-type (WT) and Kv4.2 knockout (KO) mice probed with L28/4 (left) or N52A/42 (right) TC supe.
array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/4 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford). Adult rat hippocampus immunohistochemistry.
Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-CASPR2 Antibody
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Immunofluorescence staining of adult rat optic nerve with K73/20 (red) and βIV-spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistryAnti-SAP97 Antibody
Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.Adult rat brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/8 (left) or K89/41 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with untagged KChIP1, KChIP2, KChIP3, KChIP4 or Kv2.1 plasmid probed with N423/75 (left) or K55/82 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA- tagged Kir6.2 or untagged Kv2.1 plasmid probed with N366/60 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.

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