Antibodies for Neuroscience Research

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Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
Anti-Mortalin/GRP75 Antibody FL650 Conjugate
Chicken anti-SNCA (green) and TH (red) double immunostaining in Parkinson's Disease patient’s substantia nigra section. Arrows indicate SNCA positive, degenerating dopamine neurons. Arrowheads indicate SNCA negative, healthy dopamine neurons. Bar = 50 um for A-D. (Image courtesy of Dr. Curt Freed’s lab, University of Colorado Anschutz Medical Campus.)Rat brain lysate was probed with no primary antibody as a control (Lane 1) or with a 1:2,500 dilution of Chicken anti-SNCA IgY (Lane 2).
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunofluorescence labeling of adult rat hippocampus with N450/53 TC supe (red) and Hoechst nuclear stain (blue).Anti-BDNF Antibody
Adult rat pontine nucleus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Immunoblot against crude C. elegans worm extracts and brain membranes from adult rat (RBM) probed with N72/16 TC supe.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
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Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe.II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390
Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
NeuroMab Anti-BAF53b Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Immunolabeling of mouse skeletal muscle staining NaV 1.4 (Cat. 75-424, 1:1000, red) and acetylcholine receptor (red). The frozen tissue section was fixed with 4% PFA for 10 minutes and mounted before staining. Image kindly provided by Chengjie Xie and Lauren Fish, Brown University.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Adult rat brain membrane immunoblot.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat striatum immunohistochemistry.
Adult rat brain membrane immunoblot.

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