Anti-Ankyrin-G (Blotting) Antibody (N106/20)

Name: Anti-Ankyrin-G (Blotting) Antibody
Brand: NeuroMab
SKU: 75-187
Price: 337.0 USD
Availability: InStock

Our mouse Ankyrin-G (Blotting) monoclonal antibody from NeuroMab is produced in-house from clone N106/20. It detects human, mouse, non-human primate, and rat Ankyrin-G (Blotting) and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (Cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554

Representative field of view (FOV) for near-simultaneous recording of calcium activity (Fluo-4) and axonal mitochondrial transport (mitoTracker Red) of primary mouse hippocampal neurons in sparse cultures. Arrows mark axonal fiber. Inset: MAP2 (green), AnkG (magenta) and Tau immunolabeling to verify axonal identity and origin. Arrow indicates axon initial segment. Dotted line indicates axonal path. image from publication, CC-BY-4.0. PMID: 39698051.

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 75-187

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ankyrin-G (Blotting)

Scientific Background

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N106/20

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

ANK3

Molecular Weight

>200 kDa

Immunogen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2057718

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.115

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ANKYRIN-G; Ankyrin-3; ANK-3; ankyrin 3; node of Ranvier (ankyrin G

UniProt ID

E9PE32
UniProt Summary: Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. In skeletal muscle, required for costamere localization of DMD and betaDAG1. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption. Required for intracellular adhesion and junctional conductance in myocytes, potentially via stabilization of GJA1/CX43 protein abundance and promotion of PKP2, GJA1/CX43, and SCN5A/Nav1.5 localization to cell-cell junctions.

NCBI Gene ID

288

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Ankyrin-G (Blotting) Antibody (N106/20)

SKU: 75-187

Product Details

Bulk Order Anti-Ankyrin-G (Blotting) Antibody

Related Products

Recently Viewed