Antibodies for Neuroscience Research

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Adult rat brain membrane immunoblotRepresentative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat. 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-C3 and –A3 and untagged Kv2.1 plasmids and probed with N174B/27 (left) and N159/5 (right) TC supe.
Adult rat brain membrane immunoblot.
Crude lysate of 7-day old zebrafish larvae immunoblot.Isolated goldfish cone immunohistochemistry. Data courtesy of Diane Henry-Vanisko and Gary Matthews.
Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cortex layer 2/3 with L115/13 (green), GABA (red) and DAPI (blue). Volume reconstruction of 35 serial 70 nm sections show two NPY-positive neurons with varying levels of GABA reactivity, as well as some NPY-reactive axonal varicosities. Images courtesy of Kristina Micheva (Stanford).Adult rat cortex immunohistochemistry.
Cerebellum immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot on adult rat brain membrane (RBM) and lysates from Kvβ1.1 -transfected (COS+) and untransfected (COS-) COS cells.
Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with Myc-tagged Kv2.2short, GFP-tagged Kv2.2long or untagged Kv2.1 plasmid probed with K37/89 (left), K89/34 (middle) or N52A/42 (right) TC supe.
Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of cultured mouse caudate neurons showing punctate distribution of synapsin (Cat. 1925-SYNP, 1:1000, green) and MAP (red). Cells and photo courtesy of QBMCellScience.

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