Antibodies for Neuroscience Research

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Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Aves Labs Anti-C-MYC Epitope Tag Antibody
$311
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
NeuroMab Anti-HCN2 Antibody
$394
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).Adult rat spinal cord dorsal horn immunohistochemistry.
NeuroMab Anti-TrpV1 Antibody
$337
Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling 3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Bassoon Antibody
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Anti-ChAT (Choline Acetyltransferase) AntibodyAnti-ChAT (Choline Acetyltransferase) Antibody
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Immunoblot against brain membranes from adult rat (RBM), Slo2.2 knockout (MBM-Slo2.2 KO) or wild-type (MBM-WT) mice probed with N3/26 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Chris Lingle (Washington University).Double immunofluorescence staining revealed the expression of the Slack channel (A,D, in red) in somatostatin positive neurons (B,E, in green). Image from publication CC-BY-4.0. PMID:35359569
NeuroMab Anti-Slo2.2/Slack K+ Channel Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1472) Antibody
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Western blot of HEK293 lysate showing specific immunolabeling of the ~160 kDa MerTK phosphorylated at Tyr749/753/754 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 60 minutes).Western blot image of human THP-1 cells treated with γ-Carboxylated Gas6 at a 10 nM concentration showing increased MerTK phosphorylation (Cat. p186-749). Image from publication CC-BY-4.0. PMID: 38673989
PhosphoSolutions Anti-MerTK (Tyr749/753/754) Antibody
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Adult rat hippocampus immunohistochemistry.Immunoblot versus adult rat brain membranes (RBM), and adult mouse hippocampal membranes (MHM) from SAP102 knockout and WT mice (mouse samples courtesy of Dr. Richard Huganir).
NeuroMab Anti-SAP102 Antibody
$337
Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.
NeuroMab Anti-Aldh1L1 (Blotting) Antibody
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Immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).Western blot showing specific labeling of PSD95 (Cat. 75-348) in wild type (WT) and Mir324 KO mouse hippocampus. Image from publication CC-BY-4.0. PMID:38082035
NeuroMab Anti-PSD-95 MAGUK Scaffold Protein Antibody
$394
Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (Cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0.
PhosphoSolutions Anti-CaM Kinase II (Thr286) Antibody
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Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Mortalin/GRP75 Antibody
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Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.
Aves Labs Anti-DYKDDDDK Epitope Tag/FLAG Antibody
$311
Immunofluorescence staining of sections from adult GluN2C/NR2C wild-type (WT) and knockout (KO) mouse cerebellum with N422/18 (GluN2C/NR2C, red) and L106/83 (Gephyrin, green) TC supe. Tissue courtesy of Sharon Swanger and Stephen Traynelis (Emory).Adult rat brain membrane immunoblot.
NeuroMab Anti-GluN2C/NR2C Antibody
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Adult rat cerebral cortex immunohistochemistry.Hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment showing axon initial segment and nodal staining.
NeuroMab Anti-Beta4-Spectrin Antibody
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Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cerebellum with L109/57 (red) and DAPI (blue). Left panel is a single 70 nm section imaged with a 20X objective, with a scale bar of 50 µm. Right panel is a MAX projection of 10 serial 70 nm sections imaged with a 63X objective, with a scale bar of 20 µm. Images courtesy of Kristina Micheva (Stanford).Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-CALB1/calbindin Antibody
$337
Immunofluorescence of COS7 cells expressing mKate2 or mRuby using chicken anti-mKate2 antibody (green) and mKate2 autofluorescence (red), showing complete overlap in the signal in transfected cells. The cells were stained using goat anti-chicken IgY FITC secondary antibody (Cat. 80-1000-FITC) and mounted with Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. Western blotting of mock, mKate2, or mRuby transfected COS-7 cell lysates with Aves Labs chicken anti-mKate2 antibody (0.2 ug/ml) and detected with anti-chicken HRP secondary antibody (Cat. 80-1000-HRP). Aves Labs chicken anti-mKate2 recognizes exogenous mKate2 as well as mRuby, another fluorescent protein derived from Entacmaea quadricolor in COS-7 cells.
Aves Labs Anti-mKate2 Antibody
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Representative image of 4% paraformaldehyde fixed free-floating mouse cerebellum sections stained with Aves Labs anti-PSD-95 recombinant chicken monoclonal antibody (red, Cat No. 78-028) at 1:1000 dilution and co-stained with anti-Calretinin rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum. Image courtesy EnCor Biotechnology.Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of PSD-95. The sections were stained with anti-PSD-95 recombinant chicken monoclonal antibody (green)  (Cat. 78-028) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.​ Magnification 100X.
Aves Labs Anti-PSD-95 Recombinant Chicken Chimeric mAb
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71 kDa FMRP protein phosphorylated at Ser499 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-FMRP (Ser499) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr306 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 minutes).
PhosphoSolutions Anti-CaM Kinase II (Thr306) Antibody
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Immunostaining of HeLa cells showing specific labeling of Ki-67 (Cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.
Aves Labs Anti-Ki-67 Antibody
$301

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