Anti-ChAT (Choline Acetyltransferase) Antibody

Our goat ChAT (Choline Acetyltransferase) polyclonal antibody from PhosphoSolutions is produced in-house. It detects avian, chicken, guinea pig, human, mouse, non-human primate, opossum, rat, and zebrafish ChAT (Choline Acetyltransferase) and is affinity purified. Works in ELISA, WB, IHC.

Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Gel was loaded with Mouse brain lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).
Immunohistochemistry detection of ChAT in Coronal section of rat neocortex tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. 315-CHAT) was incubated with the sample. 315-CHAT showing the choline acetyltransferase positive cholinergic nerve fibers and terminals. Reference: D. Anandh, K Shobha and Dr. Bindu M Kutty, Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Bangalore, India.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.
Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Gel was loaded with NIH/3T3 lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via ECL using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).
Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Gel was loaded with A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Human Placenta lysate (Lane 6), Huvec lysate (Lane 7), Jurkat lysate (Lane 8), L6 lysate (Lane 9), Mouse brain lysate (Lane 10), NIH/3T3 lysate (Lane 11), PC12 lysate (Lane 12), PC3 lysate (Lane 13), RAW264.7 lysate(Lane 14. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).
Immunohistochemistry detection of ChAT in rat organotypic slice cultures of septum tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. 315-CHAT) was incubated with the sample at 1:100.
Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Gel was loaded with Mouse brain lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).
Western blot detection of ChAT using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 315-CHAT). Gel was loaded with Mouse brain lysate. Proteins were transferred to the PVDF membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample at 1:1000 and then detected via chemiluminescence using rabbit anti-goat secondary antibody conjugated to HRP. Arrow indicates protein ChAT (~68-70 kDa).
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Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat
ELISA, IHC, WB
Goat

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SKU: 315-ChAT

Volume: 100 µL
Price:
$456.00
Ships: 1-2 business days

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