Anti-GluN2C/NR2C Antibody (N422/18)

Name: Anti-GluN2C/NR2C Antibody
Brand: NeuroMab
SKU: 75-411
Price: 337.0 USD
Availability: InStock

Our mouse GluN2C/NR2C monoclonal antibody from NeuroMab is produced in-house from clone N422/18. It detects human, mouse, and rat GluN2C/NR2C and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, ELISA.

Immunofluorescence staining of sections from adult GluN2C/NR2C wild-type (WT) and knockout (KO) mouse cerebellum with N422/18 (GluN2C/NR2C, red) and L106/83 (Gephyrin, green) TC supe. Tissue courtesy of Sharon Swanger and Stephen Traynelis (Emory).

$Immunoblot versus crude membrane fractions from GluN2C/NR2C knockout (KO) and wild-type (WT) mouse cerebella probed with N422/18 (top) and tubulin (bottom). Data courtesy of Bo-Shiun Chen (Augusta University) and reproduced with permission from Scientific Reports (2016 Chung et al, PMID 27845401).$

Immunoblot from NAc synaptoneurosomal preparation of WT and GluN2C KO mice, confirming loss of GluN2C subunit. Image from publication CC-BY-4.0. PMID: 35867797

Immunofluorescence staining of sections from adult GluN2C/NR2C wild-type (WT) and knockout (KO) mouse cerebellum with N422/18 (GluN2C/NR2C, red) and L106/83 (Gephyrin, green) TC supe. Tissue courtesy of Sharon Swanger and Stephen Traynelis (Emory).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-411

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN2C/NR2C

Scientific Background

Glutamate receptor ionotropic, NMDA 2C, Nmethyl D-aspartate receptor subtype 2C, or GluN2C/NR2C is encoded by the gene GRIN2C. GluN2C is a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of channel forming ionotropic glutamate receptor which is permeable to cations including calcium, potassium and sodium. GluN2C is found in the central nervous system and the cerebellum. GluN2C is thought to have an important role in physiological processes such as learning, memory, and synaptic development. Diseases associated with this gene include Alzheimer disease, Parkinson’s disease and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N422/18

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Grin2c

Molecular Weight

130 kDa

Immunogen

Fusion protein amino acids 836-1233 (cytoplasmic C-terminus) of rat GluN2C (accession number Q00961) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2531892

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

GluN2C; Glutamate receptor ionotropic; Glutamate [NMDA receptor subunit epsilon-3; GluN2C; Glutamate Receptor Ionotropic N Methyl D Aspartate 2C; Glutamate [NMDA receptor subunit epsilon-3; Glutamate receptor ionotropic NMDA 2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; Grin2c; NR2C; Grin 2c; N Methly D Aspartate Receptor Channel Subunit Epsilon 3; N Methyl D Aspartate Receptor Subtype 2C; N methyl D aspartate receptor subunit 2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NMDE3_HUMAN; NR2C; OTTHUMP00000041930; NMDA 2C

UniProt ID

Q00961
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). Participates in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current and long-term synaptic potentiation. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.

NCBI Gene ID

24411

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Anti-GluN2C/NR2C Antibody (N422/18)

SKU: 75-411

Product Details

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