Anti-Mortalin/GRP75 Antibody (N52A/42)

Name: Anti-Mortalin/GRP75 Antibody
Brand: NeuroMab
SKU: 75-127
Price: 337.0 USD
Availability: InStock

Our mouse Mortalin/GRP75 monoclonal antibody from NeuroMab is produced in-house from clone N52A/42. It detects C. elegans, human, mouse, non-human primate, and rat Mortalin/GRP75 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude C. elegans worm extracts and RBM probed with N52A/42 TC supe.

Immunoblot against HEK293 cells transfected as shown. Note that GRP75 is endogenously expressed in HEK (and COS) cells and the relatively weak RBM staining is due to short film exposure time. Cell samples courtesy of Gino Cortopassi (UC Davis).

$Western blot showing immunoblotting of cytochrome c oxidase I (COXI), cytochrome c oxidase IV (COXIV), GRP75/mortalin, VDAC1, MFN2 , and calnexin in various mouse whole brain fraction. ER (endoplasmic reticulum); CM (crude mitochondria); PM (pure mitochondria); MAM (mitochondria-associated membranes). Image from publication CC-BY-4.0. PMID: 35943566$

Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

Click on image to zoom

Species Reactivity

C. elegans, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-127

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Mortalin/GRP75

Scientific Background

Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N52A/42

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Hspa9 Grp75 Hsp74 Hspa9a

Molecular Weight

75 kDa

Immunogen

Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Immunogen Species

Mouse

Species Reactivity

C. elegans, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2120479

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.54

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

74kDa; Stress-70 protein; Grp75; 75 kDa glucose-regulated protein; Hsc74; GRP-75; Hsp74; Heat shock 70 kDa protein 9; Hsp74a; Mortalin; Hspa9a; Peptide-binding protein 74; PBP74; Mot-2; p66 MOT; Mot2; Mthsp70; mitochondrial; C3H-specific antigen; heat shock protein 9A; heat shock protein cognate 74; heat shock protein; 74 kDa

UniProt ID

P38647
UniProt Summary: Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones. In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins. Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. Regulates mitochondrial calcium-dependent apoptosis by coupling two calcium channels, ITPR1 and VDAC1, at the mitochondria-associated endoplasmic reticulum (ER) membrane to facilitate calcium transport from the ER lumen to the mitochondria intermembrane space, providing calcium for the downstream calcium channel MCU, which releases it into the mitochondrial matrix. Although primarily located in the mitochondria, it is also found in other cellular compartments. In the cytosol, it associates with proteins involved in signaling, apoptosis, or senescence. It may play a role in cell cycle regulation via its interaction with and promotion of degradation of TP53. May play a role in the control of cell proliferation and cellular aging. Protects against reactive oxygen species (ROS). Extracellular HSPA9 plays a cytoprotective role by preventing cell lysis following immune attack by the membrane attack complex by disrupting formation of the complex.

NCBI Gene ID

15526

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Anti-Mortalin/GRP75 Antibody (N52A/42)

SKU: 75-127

Product Details

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