Anti-Bassoon Antibody (L124/59)

Name: Anti-Bassoon Antibody
Brand: NeuroMab
SKU: 75-491
Price: 337.0 USD
Availability: InStock

Our mouse Bassoon monoclonal antibody from NeuroMab is produced in-house from clone L124/59. It detects mouse and rat Bassoon and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA, AT.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling 3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box.

Adult rat hippocampus immunohistochemistry.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling 3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 75-491

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Bassoon

Scientific Background

Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L124/59

Isotype

IgG2b

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Bsn

Molecular Weight

400 kDa for full-length protein, many smaller bands possible depending on sample used

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2744564

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ZNF231; Protein bassoon; neuronal double zinc finger protein; zinc finger protein 231

UniProt ID

O88778
UniProt Summary: Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released. After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts. At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. Also functions in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5. Also mediates synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import. Inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission.

NCBI Gene ID

8927

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Bassoon Antibody (L124/59)

SKU: 75-491

Product Details

Bulk Order Anti-Bassoon Antibody

Related Products

Recently Viewed