Anti-QKI-7 Antibody (N183/15)

Name: Anti-QKI-7 Antibody
Brand: NeuroMab
SKU: 75-200
Price: 337.0 USD
Availability: InStock

Our mouse QKI-7 monoclonal antibody from NeuroMab is produced in-house from clone N183/15. It detects human, mouse, and rat QKI-7 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b and untagged Kv2.1 plasmids and probed with N183/15 (left) and mouse anti-Flag (right.

Western blot of protein extracted from UD hESCs, DE cells, and CM cells probed with antibodies for QKI5 (magenta, top panel) and panQKI (green), QKI6 (magenta, middle panel) and panQKI (green), or QKI7 (magenta, bottom panel; ND denotes not detectable) and panQKI (green). Image from publication CC-BY-4.0. PMID: 35544276

Double immunofluorescence revealing pruned Lewy Neurites (pSyn, Red) and the cytoplasms of mature mouse OLs (QKI-7, cat. 75-200, 1:500; green) at the level of the anterior commissure of a mouse 6 months after the intrastriatal injection of α-Syn PFFs. Horizontal brain section. Nuclei are counterstained with DRAQ7 (white). Image from publication CC-BY-4.0. PMID:36830639

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 75-200

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

QKI-7

Scientific Background

QKI7 is a member of the Signal Transduction and Activation of RNA proteins (STAR proteins) family. QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. QKI7 is a RNA-binding protein. QKI6 has roles in the regulation of splicing, export and stability of mRNA. It is involved in myelinization. It is a cytoplasmic isoform. Diseases associated with this gene include Angiocentric Glioma, 6Q Terminal Deletion Syndrome and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N183/15

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Qki Qk QKI HKQ Qk1 Qka1

Molecular Weight

36-38 kDa

Immunogen

Synthetic peptide amino acids 303-317 (LEWIEMPVMPDISAH, C-terminal tail) of human QKI-7 (accession number Q96PU8-5)

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2173148

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

Does not cross-react with QKI-5, QKI-6 or QKI-7b

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Hqk; Protein quaking; QK1; QK3; HqkI; QKI/LOC100132735 fusion; RNA binding protein HQK; quaking homolog; KH domain RNA binding

UniProt ID

Q96PU8
UniProt Summary: RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/or translation. Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, glial cell fate and development. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G). Promotes the formation of circular RNAs (circRNAs) during the epithelial to mesenchymal transition and in cardiomyocytes: acts by binding to sites flanking circRNA-forming exons. CircRNAs are produced by back-splicing circularization of pre-mRNAs. Plays a central role in myelinization via 3 distinct mechanisms. First, acts by protecting and promoting stability of target mRNAs such as MBP, SIRT2 and CDKN1B, which promotes oligodendrocyte differentiation. Second, participates in mRNA transport by regulating the nuclear export of MBP mRNA. Finally, indirectly regulates mRNA splicing of MAG pre-mRNA during oligodendrocyte differentiation by acting as a negative regulator of MAG exon 12 alternative splicing: acts by binding to HNRNPA1 mRNA splicing factor, preventing its translation. Involved in microglia differentiation and remyelination by regulating microexon alternative splicing of the Rho GTPase pathway. Involved in macrophage differentiation: promotes monocyte differentiation by regulating pre-mRNA splicing in naive peripheral blood monocytes. Acts as an important regulator of muscle development: required for the contractile function of cardiomyocytes by regulating alternative splicing of cardiomyocyte transcripts. Acts as a negative regulator of thermogenesis by decreasing stability, nuclear export and translation of mRNAs encoding PPARGC1A and UCP1. Also required for visceral endoderm function and blood vessel development. May also play a role in smooth muscle development. In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2.

NCBI Gene ID

9444

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-QKI-7 Antibody (N183/15)

SKU: 75-200

Product Details

Bulk Order Anti-QKI-7 Antibody

Related Products

Recently Viewed