Anti-Synapsin (Ser549) Antibody

Name: Anti-Synapsin (Ser549) Antibody
Brand: PhosphoSolutions
SKU: p1560-549
Price: 456.0 USD
Availability: InStock

Our Anti-Synapsin (Ser549) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Synapsin (Ser549) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser549 (Cat. p1560-549, green, 1:500). Cells and photo courtesy of QBMCellScience.

In vivo inhibition of Cdk5 assessed via quantitative immunoblot for P-Ser549 (Cat. p1560-549)/total Synapsin I in rat hippocampus after treatment with 50 mg/kg 25–106. Image from publication CC-BY-4.0. PMID: 36854738

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Click on image to zoom

Species Reactivity

Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1560-549

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Synapsin (Ser549)

Scientific Background

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser-549 along with Ser-62 and Ser-67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate

Antibody Registry ID

AB_2492246

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser549

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI

UniProt ID

P17599
UniProt Summary: Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity). {ECO:0000250|UniProtKB:O88935, ECO:0000250|UniProtKB:P09951, ECO:0000269|PubMed:21441247, ECO:0000269|PubMed:23406870}.

NCBI Gene ID

281510

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Blue Ice

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Anti-Synapsin (Ser549) Antibody

SKU: p1560-549

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