Primary Antibodies

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Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.
Immunoblot against bacterially expressed Thioredoxin (TRX) and/or 6xHistidine (His) tagged fusion proteins.
Immunolabeling of mouse skeletal muscle staining NaV 1.4 (Cat. 75-424, 1:1000, red) and acetylcholine receptor (red). The frozen tissue section was fixed with 4% PFA for 10 minutes and mounted before staining. Image kindly provided by Chengjie Xie and Lauren Fish, Brown University.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged TARPGamma2/3/4/8 or untagged Kv2.1 plasmid and probed with N245/36 (left) and N86/8 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).Immunoblot versus adult rat brain membranes (RBM) and adult mouse brain membranes from wild-type (WT) and SynCAM4 knockout (KO) mice and probed with N244/5 (left) and K89/41 (right) TC supe. Samples courtesy of Matt Rasband, Baylor College of Medicine.
Cultured rat hippocampal neuron immunofluorescence: N23B/49 (Pan-Shank) = red, rabbit anti-PSD-95 = green, merge = yellow (bottom right). Image courtesy of Dr. Albert Hung, Picower Institute, MIT.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Pan-Shank Antibody
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Immunoblot versus low-speed pellet samples from wild-type (WT) and Stargazer mutant (STG) mice and probed with N245/1 TC supe. Data courtesy of Susumu Tomita, Yale University.
Adult rat brain membrane (RBM) immunoblot.
Adult rat brain membrane (RBM) immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Tissue and transfected cell immunoblot: low speed pellet samples from adult rat brain (RBLSP) and extracts of COS cells transiently transfected with Myc-tagged PEX6 or untagged Kv2.1 plasmids and probed with N233/8 (left) and K89/34 (right).
Adult rat hippocampus (top), thalamus (bottom left) and superior colliculus (bottom right) immunohistochemistry with antigen retrieval via pepsin pretreatment.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with DsRed-tagged GABAARAlpha4, Flag-tagged GABAARAlpha6 or untagged Kv2.1 plasmid probed with N229A/32 (left), Mouse anti-Flag (middle) or N398A/34 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Lhx6.1 and untagged Kv2.1 plasmids and probed with N228A/16 (left) and 19E10 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Pex7 or untagged Kv2.1 plasmid and probed with N232/9 (left) and 19E10 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged FGF13B/FHF2B, FGF13A/FHF2A or untagged Kv2.1 plasmid and probed with N225A/10 (left) or N86/8 (right) TC supe.
Adult rat brain immunohistochemistry.
Adult rat brain membrane immunoblot.Transfected cell immunofluorescence staining N22/21=green, rabbit anti-Shank1=red, DAPI=blue.
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).Adult rat spinal cord dorsal horn immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N227/21 (left) and loading control (right) TC supe.

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