Anti-LRRK2/Dardarin, C-Terminus Antibody (N241A/34)

Name: Anti-LRRK2/Dardarin, C-Terminus Antibody
Brand: NeuroMab
SKU: 75-253
Price: 394.0 USD
Availability: InStock

Our mouse Dardarin/LRRK2, C-terminus monoclonal antibody from NeuroMab is produced in-house from clone N241A/34. It detects drosophila, human, mouse, non-human primate, and rat Dardarin/LRRK2, C-terminus and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

iPSC-DA neuronal cultures from control and LRRK2R1441C patient lines were treated with DMSO or CCCP (24 h, 10 μM) then fixed, stained for LRRK2 (Cat. 75-253, 1:100), TOM20 and MIRO1. Image from publication CC-BY-4.0. PMID:37384414

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with N241A/34 (left) and K89/41 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson (Johns Hopkins University).

Representative Western blotting of total LRRK2, LRRK2-pSer935, pRab10, Rab10 total, and GAPDH levels following treating human peripheral blood mononuclear cells (PBMCs) with XL01126, a LRRK2 degrader, and cis-XL01126, a non-degrader control, at the indicated concentrations for 4 h. Image from publication CC-BY-4.0. PMID: 36007011

Western blot of cells isolated from the spleen of LRRK2 WT and KO mice showing specific immunolabeling of LRRK2 protein (Cat. 75-253, 1:1000). Anti-Alpha-tubulin and MODE-K lysate were used as controls. The image was kindly provided by Rebecca Pemberton from the Swamy Lab, University of Dundee, United Kingdom.

Representative western blot of WT-LRRK2 or G2019S-LRRK2 cells treated with DMSO, 10 nM, 100 nM or 1 µM MLi-2 for 2 h and assessed for LRRK2 pSer935 and full-length LRRK2 (Cat. 75-253, 1:5000). Image from publication, CC-BY-4.0. PMID: 38429321.

$Chemiluminescent immunoblot of endogenous LRRK2 (Cat. 75-253) in cytoplasmic and membrane fractions of B16 melanocytes with Rab38 versus control siRNA knockdown. TFRC and GAPDH are used as membrane and cytoplasmic markers, respectively. Image from publication CC-BY-4.0. PMID:37625589$

iPSC-DA neuronal cultures from control and LRRK2R1441C patient lines were treated with DMSO or CCCP (24 h, 10 μM) then fixed, stained for LRRK2 (Cat. 75-253, 1:100), TOM20 and MIRO1. Image from publication CC-BY-4.0. PMID:37384414

Click on image to zoom

Species Reactivity

Drosophila, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 75-253

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Dardarin/LRRK2, C-terminus

Scientific Background

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N241A/34

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 970-2527 (C-terminus) of human LRRK2 (accession number Q5SS00) produced recombinantly in E. Coli.

Immunogen Species

Human

Species Reactivity

Drosophila, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_10675136

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt ID

Q5S007

Shipping

Shipped on ice packs

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Anti-LRRK2/Dardarin, C-Terminus Antibody (N241A/34)

SKU: 75-253

Product Details

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