Anti-SynCAM4 Antibody (N244/5)

Name: Anti-SynCAM4 Antibody
Brand: NeuroMab
SKU: 75-247
Price: 337.0 USD
Availability: InStock

Our mouse SynCAM4 monoclonal antibody from NeuroMab is produced in-house from clone N244/5. It detects human, mouse, and rat SynCAM4 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).

Immunoblot versus adult rat brain membranes (RBM) and adult mouse brain membranes from wild-type (WT) and SynCAM4 knockout (KO) mice and probed with N244/5 (left) and K89/41 (right) TC supe. Samples courtesy of Matt Rasband, Baylor College of Medicine.

Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-247

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SynCAM4

Scientific Background

SynCAM4 cell adhesion molecule, Immunoglobulin Superfamily, Member 4C (IGSF4C), or Nectin-Like Protein 4 (NECL4) is encoded by the gene CADM4 and is a member of the Immunoglobulin-like Cell Adhesion Molecules family. SynCam4 is a cell adhesion molecule involved in many cell processes including the mediation of cell-cell adhesion and assembly, neuronal migration axon growth and differentiation. SynCAM1 is expressed in several tissue types including brain, kidney and liver. Diseases associated with with this gene include Small Intestine Adenocarcinoma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N244/5

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Cadm4 Igsf4c Necl4 Tsll2

Molecular Weight

43 kDa

Immunogen

Fusion protein amino acids 1-321 (signal peptide and extracellular N-terminus) of mouse SynCAM4 (accession number Q8R464) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10676101

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with other SynCAMs (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Cell adhesion molecule 4 (Immunoglobulin superfamily member 4C) (IgSF4C) (Nectin-like protein 4) (NECL-4) (TSLC1-like protein 2)

UniProt ID

Q8R464

Shipping

Shipped on ice packs

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Anti-SynCAM4 Antibody (N244/5)

SKU: 75-247

Product Details

Bulk Order Anti-SynCAM4 Antibody

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