Our mouse Shank2 monoclonal antibody from NeuroMab is produced in-house from clone N23B/6. It detects human, mouse, rabbit, and rat Shank2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.
SH3 and multiple ankyrin repeat domains protein 2, or shank 2 is a member of the shank family. Shank 2 binding domains include a SH3 domain, a proline rich domain, and a PDZ domain. Shank 2 is found in kidney, liver tissue, and epithelial cells. In brain, Shank 2 is expressed at the apical cell membrane, cytoplasm, synapse, postsynaptic density, growth cone, and dendritic spine. Some isoforms of shank 2 are expressed during development, while others are expressed in mature tissue. Shank 2 acts as a scaffold protein anchoring and connecting membrane bound receptors, including NMDA and glutamate receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 2 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 2 gene have been associated with autism spectrm disorder and blount disease.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N23B/6
IgG1
ELISA, ICC, IHC, IP, WB
Mouse
Shank2 Cortbp1
160 kDa
Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (accession number Q9QX74) produced recombinantly in E. Coli
Rat
Human, Mouse, Rabbit, Rat
AB_2254586
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.18
WB: 1:1000
IHC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity against Shank1 or Shank3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3)
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-088-n23b-6-wb-2_120x120.jpg?v=1626904249)
