Anti-SAP97 Antibody (K64/15)

Name: Anti-SAP97 Antibody
Brand: NeuroMab
SKU: 75-030
Price: 337.0 USD
Availability: InStock

Our mouse SAP97 monoclonal antibody from NeuroMab is produced in-house from clone K64/15. It detects human, mouse, rabbit, and rat SAP97 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, IP, ELISA.

Adult rat hippocampus immunohistochemistry

Immunoblot versus brain lysates from SAP97 wild-type (WT), knockout (KO) and heterozygous (Het) adult mice using K64/15 pure at 1 µg/mL (data courtesy of Lauren Makuch and Dr. Richard Huganir, Johns Hopkins University School of Medicine).

Immunoblots on brain membranes prepares from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)]

Adult rat hippocampus immunohistochemistry

Click on image to zoom

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ELISA, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-030

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SAP97

Scientific Background

Synapse-associated protein 97, Discs Large MAGUK Scaffold Protein 1 or SAP97 is encoded by the gene DLG1. SAP97 is a member of the MAGUK (membrane-associated guanylate kinase) protein family. It is a multi-domain scaffolding protein which serves to recruit receptors channels and other signaling molecules to the plasma membrane. SAP97 is expressed in heart, lung, epithelial cells and brain. SAP97 may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Diseases associated with DLG1 include Mayer-Rokitansky-Kuster-Hauser Syndrome and Depression.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K64/15

Isotype

IgG1

Applications

ELISA, IHC, IP, WB

Host Species

Mouse

Gene Name

Dlg1 Dlgh1

Molecular Weight

130 kDa

Immunogen

Fusion protein amino acids 1-104 (N-terminus) of rat SAP97 (accession number Q62696) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2091921

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity against PSD-95, Chapsyn-110 and SAP102 expressed in transfected cells

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Dlgh1; Disks large homolog 1; Synapse-associated protein 97; SAP-97; discs; SAP97; large homolog 1; hDlg

UniProt ID

Q62696
UniProt Summary: Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane.

NCBI Gene ID

25252

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Anti-SAP97 Antibody (K64/15)

SKU: 75-030

Product Details

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