Anti-CASPR2 Antibody (K67/25)

Name: Anti-CASPR2 Antibody
Brand: NeuroMab
SKU: 75-075
Price: 337.0 USD
Availability: InStock

Our mouse CASPR2 monoclonal antibody from NeuroMab is produced in-house from clone K67/25. It detects human, mouse, and rat CASPR2 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.

Adult rat hippocampus immunohistochemistry.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)].

$Western blots of tissue fractionation of mouse cortex demonstrate the presence of smaller shed ectodomains of CNTNAP2 (intracellular),CNTNAP2 (extracellular) (Cat. 75-075), Neuroligin 1 and 3, and Neuropilin 2 in S2 fractions. Image from publication CC-BY-4.0. PMID:38962061$

Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-075

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CASPR2

Scientific Background

Contactin-associated protein-like 2 protein, or Caspr2, is a neuronal transmembrane member of the Neurexin family. Structural features of Caspr2 include epidermal growth factor repeats, laminin G domains, F5/8 type C domain, fibrinogen-like domains, and PDZ binding sites. Caspr2 is a cell adhesion molecule as well as a receptor. It is involved in mediating cell–cell interactions in the nervous system and involved in neural-glia interactions and clustering of potassium channels in myelinated axons. It is expressed at the juxaperanodal region of nodes of Ranvier in the CNS and PNS. Changes in expression have been associated with increased susceptibility to autism and epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K67/25

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CNTNAP2 hCG_15499 tcag7.496

Molecular Weight

180 kDa

Immunogen

Fusion protein 96-1265 (extracellular domain) of human Caspr2 (accession number A0A090N7T7) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2245198

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.7

Dilution Ranges

WB: 1:500
IHC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against Caspr/paranodin

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

AUTS15; Contactin associated protein-like 2; CDFE; NRXN4; PTHSL1; contactin-associated protein-like 2; cell recognition molecule Caspr2; homolog of Drosophila neurexin IV; isoform CRA_b

UniProt ID

A0A090N7T7
UniProt Summary: Required for gap junction formation (Probable). Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction.

NCBI Gene ID

26047

Shipping

Shipped on ice packs

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Anti-CASPR2 Antibody (K67/25)

SKU: 75-075

Product Details

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