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"Endogenous and transfected cell immunoblot: extracts of HEK-293 cells transfected with GFP-tagged LRRK2-WT, LRRK2-S910A or LRRK2-S935A plasmid; fibroblasts from embryonic WT or LRRK2 KO mice untreated or treated with LRRK2-IN-1 inhibitor (2011 Deng et al Nat Chem Biol); and human lymphoblasts untreated or treated with LRRK2-IN-1, probed with 1D8. Data courtesy of Nicolas Dzamko, Paul Davies and Dario Alessi (University of Dundee, Scotland, UK)."
Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain membrane immunoblot.
Western Blot showing specific immunolabeling of Ly-6K in KK47 human bladder carcinoma cells, LY-6K siRNA, but not scrambled siRNA knocked down Ly-6K expression. A GAPDH antibody was used as a protein loading control. Data courtesy of Masuda et al.Western Blot of Ly6K protein with GST-tag, non-transfected control HEK293T cells, HEK293T cells transfected with Ly6K-GFP construct showing specific immunolabeling of Ly6K.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) or untagged Kv2.1 plasmid and probed with N129/15 (left) and K89/41 (right) TC supes.

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