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Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) and untagged Kv2.1 plasmids and probed with N129A/6 (left) and K89/41 (right) TC supes.
Immunoblot against extracts of COS cells transiently transfected with Flag-tagged Malin or untagged Kv2.1 plasmid probed with N85/18 TC supe (left) or rabbit polyclonal (right).
Bacterial fusion protein immunoblot.
Western Blot of 293T cell lysate showing specific immunolabeling of MAP1.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).

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