Anti-GSK-3α/β (Tyr-279/Tyr-216), Phosphospecific Antibody (M132)

Name: Anti-GSK-3α/β (Tyr-279/Tyr-216), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: GM1321
Price: 348.0 USD
Availability: InStock

Our GSK-3α/β (Tyr-279/Tyr-216) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GSK-3α/β (Tyr-279/Tyr-216) and is Protein A purified. It is great for use in WB.

Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3β (lanes 1 & 2) or anti-GSK-3α/β (Y279/Y216) (lanes 3 & 4).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: GM1321

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GSK-3α/β (Tyr-279/Tyr-216)

Scientific Background

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M132

Isotype

IgG1

Applications

ELISA, WB

Host Species

Mouse

Gene Name

GSK3A/B

Molecular Weight

46/50

Immunogen

Clone M132 was generated from a phospho-GSK-3β (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3β. This peptide sequence is also found in GSK-3α (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects 46/50 kDa* proteins corresponding to the apparent molecular mass of GSK-3β and GSK-3α on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-216

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Glycogen synthase kinase beta3

UniProt ID

P49840

Shipping

Blue Ice

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Anti-GSK-3α/β (Tyr-279/Tyr-216), Phosphospecific Antibody (M132)

SKU: GM1321

Product Details

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