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Immunohistochemistry of retinal tissue showing specific staining of Shank at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus. Neurons grown under ASD1 conditions show a significant decrease of synaptic Shank proteins, while neurons
Western Blot of rat brain lysate showing specific immunolabeling of SHANK1.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Distribution of SHANK3 immuno-positive cells in different regions of the adult mouse cerebral cortex. N, O: High-magnification views of SHANK3-positive cells in the RSPV region (N) and hippocampal region (O) as shown in image M. Green, SHANK3-positive cells (Cat no 75-344, 1:200); blue, cell nuclei. Scale bars, 300 μm (M); 100 μm (N, O). Image from publication, CC-BY-4.0. PMID: 39693031.Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
Sale priceFrom $139
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