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Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).mmunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).
Western Blot of 3 ug of recombinant SERPINB12 showing specific immunolabeling of SERPINB12.
Western Blot of 5 ug of normal oral mucosa lysate showing specific immunolabeling of SERPINB13.
Western Blot of human pancreas lysate showing specific immunolabeling of SFRP5.
Adult rat hippocampus immunohistochemistry.Adult rat whole brain immunohistochemistry.
Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Shank1 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/51 (left) or N23B/49 (right) TC supe.
Adult rat brain membrane immunoblot.Transfected cell immunofluorescence staining N22/21=green, rabbit anti-Shank1=red, DAPI=blue.

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